| Literature DB >> 26724332 |
Cong Bi1, Sandya Beeram1, Zhao Li1, Xiwei Zheng1, David S Hage2.
Abstract
Information on the kinetics of drug-protein interactions is of crucial importance in drug discovery and development. Several methods based on affinity chromatography have been developed in recent years to examine the association and dissociation rates of these processes. These techniques include band-broadening measurements, the peak decay method, peak fitting methods, the split-peak method, and free fraction analysis. This review will examine the general principles and applications of these approaches and discuss their use in the characterization, screening and analysis of drug-protein interactions in the body.Entities:
Mesh：
Substances：
Year: 2015 PMID： 26724332 PMCID： PMC4698314 DOI： 10.1016/j.ddtec.2015.09.003
Source DB: PubMed Journal: Drug Discov Today Technol ISSN： 1740-6749