| Literature DB >> 26723877 |
Hyang Sook Seol1, Sang Eun Lee1, Joon Seon Song2, Je-Keun Rhee3, Shree Ram Singh4, Suhwan Chang5, Se Jin Jang6.
Abstract
Tumor-initiating cells are important for the formation and maintenance of tumor bulks in various tumors. To identify surface markers of liver tumor-initiating cells, we performed primary tumorsphere culture and analyzed the expression of cluster of differentiation (CD) antigen genes using NanoString. Interestingly, we found significant upregulation of the complement proteins (p = 1.60 × 10(-18)), including C7 and CFH. Further studies revealed that C7 and CFH are required to maintain stemness in liver cancer cells. Knockdown of C7 and CFH expression abrogated tumorsphere formation and induced differentiation, whereas overexpression stimulated stemness factor expression as well as in vivo cell growth. Mechanistically, by studying C7 and CFH-dependent LSF-1 expression and its direct role on stemness factor transcription, we found that LSF-1 is involved in this regulation. Taken together, our data demonstrate the unprecedented role of complement proteins on the maintenance of stemness in liver tumor-initiating cells. Published by Elsevier Ireland Ltd.Entities:
Keywords: CFH; Complement protein C7; HCC; LSF-1; Liver tumor-initiating cell; Stemness factor
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Year: 2015 PMID： 26723877 PMCID： PMC4744505 DOI： 10.1016/j.canlet.2015.12.005
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679