Bin Wang1, Sufang Xiong2, Qingquan Hua1, Chen Chen1, Hua Liao1, Liu Chen1, Weiqi Yao3, Dongcheng Wu3, Zezhang Tao1. 1. Department of Otolaryngology Head and Neck Surgery, Renmin Hospital of Wuhan University Wuhan 430060, China. 2. The Fifth Hospital in Wuhan Wuhan 430000, China. 3. Department of Biochemistry and Molecular Biology, Wuhan University School of Basic Medical Sciences Wuhan 430071, China ; Wuhan Hamilton Biotechnology Co. LTD Wuhan 430075, China.
Abstract
OBJECTIVE: This study aimed to understand the relationship between tissue factor (TF) and laryngeal carcinoma. METHODS: Differences in TF expression between pericarcinomatous and carcinomatous tissues were studied in patients with laryngeal carcinoma; the potential clinical significance of the observed differences is discussed. Immunohistochemical, western blot, and RT-PCR analyses were performed to assess the expression of TF at the protein and mRNA levels, and differences between pericarcinomatous and carcinomatous tissues in patients (n = 20) with laryngeal carcinoma were analyzed. RESULTS: Expression of TF was significantly higher in pericarcinomatous tissues than in carcinomatous tissues (P < 0.01); furthermore, the intensity of TF mRNA expression was also significantly stronger in pericarcinomatous than in carcinomatous tissue (P < 0.001). Robust expression of TF was observed in pericarcinomatous tissues but not in carcinomatous tissues. CONCLUSION: TF may contribute to the carcinogenesis and development of laryngeal carcinoma and may provide a marker for assessment of the degree of malignancy and the progression of laryngeal carcinoma. TF may also provide a new target for therapeutics for human head and neck cancer.
OBJECTIVE: This study aimed to understand the relationship between tissue factor (TF) and laryngeal carcinoma. METHODS: Differences in TF expression between pericarcinomatous and carcinomatous tissues were studied in patients with laryngeal carcinoma; the potential clinical significance of the observed differences is discussed. Immunohistochemical, western blot, and RT-PCR analyses were performed to assess the expression of TF at the protein and mRNA levels, and differences between pericarcinomatous and carcinomatous tissues in patients (n = 20) with laryngeal carcinoma were analyzed. RESULTS: Expression of TF was significantly higher in pericarcinomatous tissues than in carcinomatous tissues (P < 0.01); furthermore, the intensity of TF mRNA expression was also significantly stronger in pericarcinomatous than in carcinomatous tissue (P < 0.001). Robust expression of TF was observed in pericarcinomatous tissues but not in carcinomatous tissues. CONCLUSION:TF may contribute to the carcinogenesis and development of laryngeal carcinoma and may provide a marker for assessment of the degree of malignancy and the progression of laryngeal carcinoma. TF may also provide a new target for therapeutics for human head and neck cancer.
Authors: Esther C W Breij; Bart E C G de Goeij; Sandra Verploegen; Danita H Schuurhuis; Ali Amirkhosravi; John Francis; Vibeke Breinholt Miller; Mischa Houtkamp; Wim K Bleeker; David Satijn; Paul W H I Parren Journal: Cancer Res Date: 2013-12-26 Impact factor: 12.701