Fen Ai1, Manhua Chen2, Bo Yu1, Yang Yang1, Guizhong Xu1, Feng Gui1, Zhenxing Liu1, Xiangyan Bai1, Zhen Chen1. 1. Department of Emergency, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China. 2. Department of Cardiology, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science & Technology Wuhan 430014, Hubei Province, China.
Abstract
OBJECTIVE: The traditional Chinese medicinal berberine has long been used to treat cardiovascular diseases; however, the mechanism underlying its effects remains unclear. Here, this study would to investigate the effects of berberine on proliferation, collagen synthesis and cytokine secretion of cardiac fibroblasts. METHODS: We assessed proliferation, collagen synthesis and cytokine secretion in cardiac fibroblasts subjected to angiotensin II (Ang II) subsequent to the consumption of berberine or a control treatment. And then we detected the role of AMPK/mTOR signaling pathway in berberine treatment of cardiac fibroblasts. RESULTS: In the present study, the cellular behaviors of cardiac fibroblasts induced by Ang II were significantly activated including proliferation, transformation into myofibroblasts and collagen synthesis. Additionally, the ability of cytokine secretion was enhanced obviously. It was demonstrated that treatment of cardiac fibroblasts with berberine resulted in deceased proliferation, and attenuated fibroblast α-smooth muscle actin expression and collagen synthesis. And the protein secretion of TGFβ1 was inhibited; however, the protein secretion of IL-10 was increased in cardiac fibroblasts with berberine treatment. Mechanistically, the phosphorylation level of AMPK was increased; and the phosphorylation levels of mTOR and p70S6K were decreased in berberine treatment group. CONCLUSION: These results illustrated that the protective effects of berberine on cellular behaviors of cardiac fibroblasts were at least in part due to activate AMPK signaling pathway and downregulate mTOR/p70S6K signaling pathway. Berberine might become a new strategy for treating cardiac fibrosis in the future.
OBJECTIVE: The traditional Chinese medicinal berberine has long been used to treat cardiovascular diseases; however, the mechanism underlying its effects remains unclear. Here, this study would to investigate the effects of berberine on proliferation, collagen synthesis and cytokine secretion of cardiac fibroblasts. METHODS: We assessed proliferation, collagen synthesis and cytokine secretion in cardiac fibroblasts subjected to angiotensin II (Ang II) subsequent to the consumption of berberine or a control treatment. And then we detected the role of AMPK/mTOR signaling pathway in berberine treatment of cardiac fibroblasts. RESULTS: In the present study, the cellular behaviors of cardiac fibroblasts induced by Ang II were significantly activated including proliferation, transformation into myofibroblasts and collagen synthesis. Additionally, the ability of cytokine secretion was enhanced obviously. It was demonstrated that treatment of cardiac fibroblasts with berberine resulted in deceased proliferation, and attenuated fibroblast α-smooth muscle actin expression and collagen synthesis. And the protein secretion of TGFβ1 was inhibited; however, the protein secretion of IL-10 was increased in cardiac fibroblasts with berberine treatment. Mechanistically, the phosphorylation level of AMPK was increased; and the phosphorylation levels of mTOR and p70S6K were decreased in berberine treatment group. CONCLUSION: These results illustrated that the protective effects of berberine on cellular behaviors of cardiac fibroblasts were at least in part due to activate AMPK signaling pathway and downregulate mTOR/p70S6K signaling pathway. Berberine might become a new strategy for treating cardiac fibrosis in the future.
Authors: Ronald B Driesen; Chandan K Nagaraju; Joëlle Abi-Char; Tamara Coenen; Paul J Lijnen; Robert H Fagard; Karin R Sipido; Victor V Petrov Journal: Cardiovasc Res Date: 2013-12-23 Impact factor: 10.787
Authors: Maryam Sharifi-Sanjani; Mariah Berman; Dmitry Goncharov; Mohammad Alhamaydeh; Theodore Guy Avolio; Jeffrey Baust; Baojun Chang; Ahasanul Kobir; Mark Ross; Claudette St Croix; Seyed Mehdi Nouraie; Charles F McTiernan; Christine S Moravec; Elena Goncharova; Imad Al Ghouleh Journal: Int J Mol Sci Date: 2021-06-07 Impact factor: 5.923