Ryszard Pluta1, Janusz Kocki2, Marzena Ułamek-Kozioł3, Anna Bogucka-Kocka4, Paulina Gil-Kulik2, Sławomir Januszewski3, Mirosław Jabłoński5, Alicja Petniak2, Judyta Brzozowska6, Jacek Bogucki7, Wanda Furmaga-Jabłońska8, Stanisław J Czuczwar9. 1. Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warszawa, Poland. Electronic address: pluta@imdik.pan.pl. 2. Department of Clinical Genetics, Medical University of Lublin, Lublin, Poland. 3. Laboratory of Ischemic and Neurodegenerative Brain Research, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warszawa, Poland. 4. Department of Pharmaceutical Botany, Medical University of Lublin, Lublin, Poland. 5. Department of Rehabilitation and Orthopaedics, Medical University of Lublin, Lublin, Poland. 6. Department of Clinical Psychology, Medical University of Lublin, Lublin, Poland. 7. Warsaw Higher Humanistic School, Warszawa, Poland. 8. Department of Neonate and Infant Pathology, Medical University of Lublin, Lublin, Poland. 9. Department of Pathophysiology, Medical University of Lublin, Lublin, Poland. Electronic address: czuczwarsj@yahoo.com.
Abstract
BACKGROUND: Brain ischemia may be causally related with Alzheimer's disease. Probably, presenilin gene dysregulation may be associated with Alzheimer's disease neuropathology. Consequently, we have examined quantitative changes in both presenilin 1 and 2 genes in the medial temporal lobe cortex following 10-min global brain ischemia in rats. METHODS: Global brain ischemia was induced by cardiac arrest in female rats that were allowed to survive for 2, 7 and 30 days. The expression of presenilin genes was evaluated in the rat medial temporal lobe cortex with the use of quantitative RT-PCR analysis. RESULTS: Presenilin 1 gene expression tended to be downregulated from days 2 to 7 postischemia but at day 30, there was a reverse tendency. The greatest overexpression of presenilin 2 gene was noted at 2-nd day whilst on day 7, the expression of this gene was only modestly elevated. Eventually, at day 30 expression of presenilin 2 gene was modestly downregulated. Alterations of presenilin 2 gene expression between 2 and 7 days and between 2 and 30 days were statistically significant. CONCLUSIONS: Thus, presented changes suggest that the significant dysregulation of presenilin 2 gene may be connected with a response of neuronal cells to transient global brain ischemia due to cardiac arrest. Finally, the ischemia-induced gene dysregulation may play a key role in the late onset of Alzheimer's-type dementia.
BACKGROUND:Brain ischemia may be causally related with Alzheimer's disease. Probably, presenilin gene dysregulation may be associated with Alzheimer's disease neuropathology. Consequently, we have examined quantitative changes in both presenilin 1 and 2 genes in the medial temporal lobe cortex following 10-min global brain ischemia in rats. METHODS: Global brain ischemia was induced by cardiac arrest in female rats that were allowed to survive for 2, 7 and 30 days. The expression of presenilin genes was evaluated in the rat medial temporal lobe cortex with the use of quantitative RT-PCR analysis. RESULTS:Presenilin 1 gene expression tended to be downregulated from days 2 to 7 postischemia but at day 30, there was a reverse tendency. The greatest overexpression of presenilin 2 gene was noted at 2-nd day whilst on day 7, the expression of this gene was only modestly elevated. Eventually, at day 30 expression of presenilin 2 gene was modestly downregulated. Alterations of presenilin 2 gene expression between 2 and 7 days and between 2 and 30 days were statistically significant. CONCLUSIONS: Thus, presented changes suggest that the significant dysregulation of presenilin 2 gene may be connected with a response of neuronal cells to transient global brain ischemia due to cardiac arrest. Finally, the ischemia-induced gene dysregulation may play a key role in the late onset of Alzheimer's-type dementia.
Authors: Maria Kovalska; Barbara Tothova; Libusa Kovalska; Zuzana Tatarkova; Dagmar Kalenska; Anna Tomascova; Marian Adamkov; Jan Lehotsky Journal: Neurochem Res Date: 2018-07-12 Impact factor: 3.996
Authors: Marzena Ułamek-Kozioł; Stanisław Jerzy Czuczwar; Sławomir Januszewski; Ryszard Pluta Journal: Int J Mol Sci Date: 2020-01-30 Impact factor: 5.923
Authors: Ryszard Pluta; Marzena Ułamek-Kozioł; Janusz Kocki; Jacek Bogucki; Sławomir Januszewski; Anna Bogucka-Kocka; Stanisław J Czuczwar Journal: Mol Neurobiol Date: 2019-11-12 Impact factor: 5.682