Caroline Curry Martins1, Margarete Dulce Bagatini2, Andréia Machado Cardoso1, Daniela Zanini1, Fátima Husein Abdalla1, Jucimara Baldissarelli1, Diéssica Padilha Dalenogare1, Juliano Boufleur Farinha3, Maria Rosa Chitolina Schetinger1, Vera Maria Morsch4. 1. Post-Graduation Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences of the Federal University of Santa Maria, Santa Maria, RS, Brazil. 2. Post-Graduation Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences of the Federal University of Santa Maria, Santa Maria, RS, Brazil; Collegiate of Nursing, University of Southern Frontier, Chapecó Campus, SC, Brazil. 3. Post-Graduation Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences of the Federal University of Santa Maria, Santa Maria, RS, Brazil; Physical Activity Group, Center of Physical Education and Sports, Federal University of Santa Maria, Santa Maria, RS, Brazil. 4. Post-Graduation Program in Toxicological Biochemistry, Department of Biochemistry and Molecular Biology, Center of Natural and Exact Sciences of the Federal University of Santa Maria, Santa Maria, RS, Brazil. Electronic address: veramorsch@gmail.com.
Abstract
BACKGROUND: Alterations in the activity of ectonucleotidase enzymes have been implicated in cardiovascular diseases, whereas regular exercise training has been shown to prevent these alterations. However, nothing is known about it relating to metabolic syndrome (MetS). We investigated the effect of exercise training on platelet ectonucleotidase enzymes and on the aggregation profile of MetS patients. METHODS: We studied 38 MetS patients who performed regular concurrent exercise training for 30 weeks. Anthropometric measurements, biochemical profiles, hydrolysis of adenine nucleotides in platelets and platelet aggregation were collected from patients before and after the exercise intervention as well as from individuals of the control group. RESULTS: An increase in the hydrolysis of adenine nucleotides (ATP, ADP and AMP) and a decrease in adenosine deamination in the platelets of MetS patients before the exercise intervention were observed (P<0.001). However, these alterations were reversed by exercise training (P<0.001). Additionally, an increase in platelet aggregation was observed in the MetS patients (P<0.001) and the exercise training prevented platelet hyperaggregation in addition to decrease the classic cardiovascular risks. CONCLUSIONS: An alteration of ectonucleotidase enzymes occurs during MetS, whereas regular exercise training had a protective effect on these enzymes and on platelet aggregation.
BACKGROUND: Alterations in the activity of ectonucleotidase enzymes have been implicated in cardiovascular diseases, whereas regular exercise training has been shown to prevent these alterations. However, nothing is known about it relating to metabolic syndrome (MetS). We investigated the effect of exercise training on platelet ectonucleotidase enzymes and on the aggregation profile of MetS patients. METHODS: We studied 38 MetS patients who performed regular concurrent exercise training for 30 weeks. Anthropometric measurements, biochemical profiles, hydrolysis of adenine nucleotides in platelets and platelet aggregation were collected from patients before and after the exercise intervention as well as from individuals of the control group. RESULTS: An increase in the hydrolysis of adenine nucleotides (ATP, ADP and AMP) and a decrease in adenosine deamination in the platelets of MetS patients before the exercise intervention were observed (P<0.001). However, these alterations were reversed by exercise training (P<0.001). Additionally, an increase in platelet aggregation was observed in the MetS patients (P<0.001) and the exercise training prevented platelet hyperaggregation in addition to decrease the classic cardiovascular risks. CONCLUSIONS: An alteration of ectonucleotidase enzymes occurs during MetS, whereas regular exercise training had a protective effect on these enzymes and on platelet aggregation.
Authors: Cesar Eduardo Jacintho Moritz; Alexandra Ferreira Vieira; Denise de Melo-Marins; Fabrício Figueiró; Ana Maria Oliveira Battastini; Alvaro Reischak-Oliveira Journal: Physiol Rep Date: 2022-09