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Literature DB >> 26717885

Two-step ion-exchange chromatographic purification combined with reversed-phase chromatography to isolate C-peptide for mass spectrometric analysis.

Kuanysh Kabytaev¹, Anita Durairaj¹, Dmitriy Shin¹, Curt L Rohlfing¹, Shawn Connolly¹, Randie R Little¹, Alexander V Stoyanov¹.

Abstract

A liquid chromatography with mass spectrometry on-line platform that includes the orthogonal techniques of ion exchange and reversed phase chromatography is applied for C-peptide analysis. Additional improvement is achieved by the subsequent application of cation- and anion-exchange purification steps that allow for isolating components that have their isoelectric points in a narrow pH range before final reversed-phase mass spectrometry analysis. The utility of this approach for isolating fractions in the desired "pI window" for profiling complex mixtures is discussed.

Entities: Chemical Disease Gene Species

Keywords: C-peptide; Ion exchange chromatography; Isotope dilution assay; Orthogonal separations; Peptide purification

Mesh：

Substances：
Anions
C-Peptide
Cations

Year: 2016 PMID： 26717885 PMCID： PMC6053671 DOI： 10.1002/jssc.201500989

Source DB: PubMed Journal: J Sep Sci ISSN： 1615-9306 Impact factor: 3.645

14 in total

1. An automated on-line multidimensional HPLC system for protein and peptide mapping with integrated sample preparation.

Authors: Knut Wagner; Tasso Miliotis; György Marko-Varga; Rainer Bischoff; Klaus K Unger
Journal: Anal Chem Date: 2002-02-15 Impact factor: 6.986

2. Development of an isotope dilution assay for precise determination of insulin, C-peptide, and proinsulin levels in non-diabetic and type II diabetic individuals with comparison to immunoassay.

Authors: A D Kippen; F Cerini; L Vadas; R Stöcklin; L Vu; R E Offord; K Rose
Journal: J Biol Chem Date: 1997-05-09 Impact factor: 5.157

3. Improvement of sample pretreatment prior to analysis of C-peptide in serum by isotope-dilution liquid chromatography/tandem mass spectrometry.

Authors: Diego Rodríguez-Cabaleiro; Dietmar Stöckl; Linda M Thienpont
Journal: Rapid Commun Mass Spectrom Date: 2005 Impact factor: 2.419

4. Direct sensitive quantitative lC/MS analysis of C-peptide from human urine by two dimensional reverse phase/reverse phase high-performance liquid chromatography.

Authors: Eduard Rogatsky; Vlad Tomuta; Greg Cruikshank; Louis Vele; Harsha Jayatillake; Daniel Stein
Journal: J Sep Sci Date: 2006-03 Impact factor: 3.645

5. Two-dimensional reverse phase-reverse phase chromatography: A simple and robust platform for sensitive quantitative analysis of peptides by LC/MS. Hardware design.

Authors: Eduard Rogatsky; Daniel T Stein
Journal: J Sep Sci Date: 2006-03 Impact factor: 3.645

6. Trace LC/MS quantitative analysis of polypeptide biomarkers: impact of 1-D and 2-D chromatography on matrix effects and sensitivity.

Authors: Eduard Rogatsky; Vlad Tomuta; Harsha Jayatillake; Greg Cruikshank; Louis Vele; Daniel T Stein
Journal: J Sep Sci Date: 2007-02 Impact factor: 3.645

Two-step ion-exchange chromatographic purification combined with reversed-phase chromatography to isolate C-peptide for mass spectrometric analysis.

1. An automated on-line multidimensional HPLC system for protein and peptide mapping with integrated sample preparation.

2. Development of an isotope dilution assay for precise determination of insulin, C-peptide, and proinsulin levels in non-diabetic and type II diabetic individuals with comparison to immunoassay.

3. Improvement of sample pretreatment prior to analysis of C-peptide in serum by isotope-dilution liquid chromatography/tandem mass spectrometry.

4. Direct sensitive quantitative lC/MS analysis of C-peptide from human urine by two dimensional reverse phase/reverse phase high-performance liquid chromatography.

5. Two-dimensional reverse phase-reverse phase chromatography: A simple and robust platform for sensitive quantitative analysis of peptides by LC/MS. Hardware design.

6. Trace LC/MS quantitative analysis of polypeptide biomarkers: impact of 1-D and 2-D chromatography on matrix effects and sensitivity.

Review 7. IEF-based multidimensional applications in proteomics: toward higher resolution.

8. Use of cation exchange chromatography for human C-peptide isotope dilution - mass spectrometric assay.

9. Quantitative study of insulin secretion and clearance in normal and obese subjects.

Review 10. Proinsulin and the biosynthesis of insulin.

1. Enhancing the promiscuity of a member of the Caspase protease family by rational design.

2. Development and Validation of a Novel LC-MS/MS Assay for C-Peptide in Human Serum.