Literature DB >> 26714705

HIV-1 Reverse Transcriptase-Based Assay to Determine Cellular dNTP Concentrations.

Joseph A Hollenbaugh1, Baek Kim2,3.   

Abstract

Deoxynucleoside triphosphates (dNTPs) are the building blocks of DNA and their biosynthesis is tightly regulated in the cell. HPLC-MS and enzyme-based methods are currently employed to determine dNTP concentrations from cellular extracts. Here, we describe a highly efficient, HIV-1 reverse transcriptase (RT)-based assay to quantitate dNTP concentrations. The assay is based on the ability of HIV-1 RT to function at very low dNTP concentrations, thus providing for the high sensitivity of detection.

Entities:  

Keywords:  Deoxynucleoside triphosphates; HIV-1; Reverse transcriptase; dNTP concentration

Mesh：

Substances：

Year:  2016        PMID： 26714705      PMCID： PMC4839283          DOI： 10.1007/978-1-4939-3046-3_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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