Literature DB >> 26714704

Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity.

João I Mamede1, Thomas J Hope2.   

Abstract

Live cell imaging is a valuable technique that allows the characterization of the dynamic processes of the HIV-1 life cycle. Here, we present a method of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.

Entities:  

Keywords:  HIV early steps of infection; HIV fusion; HIV uncoating; HIV viral core; Live cell imaging; Wide-field microscopy

Mesh：

Year:  2016        PMID： 26714704      PMCID： PMC4876021          DOI： 10.1007/978-1-4939-3046-3_4

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  14 in total

1.  Characterization of intracellular reverse transcription complexes of human immunodeficiency virus type 1.

Authors:  A Fassati; S P Goff
Journal:  J Virol       Date:  2001-04       Impact factor: 5.103

Review 2.  Intracellular trafficking of retroviral genomes during the early phase of infection: viral exploitation of cellular pathways.

Authors:  S P Goff
Journal:  J Gene Med       Date:  2001 Nov-Dec       Impact factor: 4.565

3.  Complementary assays reveal a relationship between HIV-1 uncoating and reverse transcription.

Authors:  Amy E Hulme; Omar Perez; Thomas J Hope
Journal:  Proc Natl Acad Sci U S A       Date:  2011-05-31       Impact factor: 11.205

4.  Envelope-dependent, cyclophilin-independent effects of glycosaminoglycans on human immunodeficiency virus type 1 attachment and infection.

Authors:  Yi-Jun Zhang; Theodora Hatziioannou; Trinity Zang; Douglas Braaten; Jeremy Luban; Stephen P Goff; Paul D Bieniasz
Journal:  J Virol       Date:  2002-06       Impact factor: 5.103

5.  Structural analyses of purified human immunodeficiency virus type 1 intracellular reverse transcription complexes.

Authors:  Milan V Nermut; Ariberto Fassati
Journal:  J Virol       Date:  2003-08       Impact factor: 5.103

Review 6.  Maturation of the HIV reverse transcription complex: putting the jigsaw together.

Authors:  David Warrilow; Gilda Tachedjian; David Harrich
Journal:  Rev Med Virol       Date:  2009-11       Impact factor: 6.989

7.  Visualization of the intracellular behavior of HIV in living cells.

Authors:  David McDonald; Marie A Vodicka; Ginger Lucero; Tatyana M Svitkina; Gary G Borisy; Michael Emerman; Thomas J Hope
Journal:  J Cell Biol       Date:  2002-11-04       Impact factor: 10.539

8.  Fusion of mature HIV-1 particles leads to complete release of a gag-GFP-based content marker and raises the intraviral pH.

Authors:  Sergi Padilla-Parra; Mariana Marin; Nivriti Gahlaut; Rolf Suter; Naoyuki Kondo; Gregory B Melikyan
Journal:  PLoS One       Date:  2013-08-09       Impact factor: 3.240

9.  Evidence for biphasic uncoating during HIV-1 infection from a novel imaging assay.

Authors:  Hongzhan Xu; Tamera Franks; Gregory Gibson; Kelly Huber; Nadia Rahm; Caterina Strambio De Castillia; Jeremy Luban; Christopher Aiken; Simon Watkins; Nicolas Sluis-Cremer; Zandrea Ambrose
Journal:  Retrovirology       Date:  2013-07-09       Impact factor: 4.602

10.  Robust single-particle tracking in live-cell time-lapse sequences.

Authors:  Khuloud Jaqaman; Dinah Loerke; Marcel Mettlen; Hirotaka Kuwata; Sergio Grinstein; Sandra L Schmid; Gaudenz Danuser
Journal:  Nat Methods       Date:  2008-07-20       Impact factor: 28.547
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  9 in total

Review 1.  Restriction of HIV-1 and other retroviruses by TRIM5.

Authors:  Barbie K Ganser-Pornillos; Owen Pornillos
Journal:  Nat Rev Microbiol       Date:  2019-07-16       Impact factor: 60.633

2.  Capsid Lattice Destabilization Leads to Premature Loss of the Viral Genome and Integrase Enzyme during HIV-1 Infection.

Authors:  Jenna E Eschbach; Jennifer L Elliott; Wen Li; Kaneil K Zadrozny; Keanu Davis; Shawn J Mohammed; Dana Q Lawson; Owen Pornillos; Alan N Engelman; Sebla B Kutluay
Journal:  J Virol       Date:  2020-12-22       Impact factor: 5.103

3.  Entry of glucose- and glutamine-derived carbons into the citric acid cycle supports early steps of HIV-1 infection in CD4 T cells.

Authors:  Isabelle Clerc; Daouda Abba Moussa; Zoi Vahlas; Saverio Tardito; Leal Oburoglu; Thomas J Hope; Marc Sitbon; Valérie Dardalhon; Cédric Mongellaz; Naomi Taylor
Journal:  Nat Metab       Date:  2019-07-12

4.  Time-Resolved Imaging of Single HIV-1 Uncoating In Vitro and in Living Cells.

Authors:  Ashwanth C Francis; Mariana Marin; Jiong Shi; Christopher Aiken; Gregory B Melikyan
Journal:  PLoS Pathog       Date:  2016-06-20       Impact factor: 6.823

Review 5.  Live-Cell Imaging of Early Steps of Single HIV-1 Infection.

Authors:  Ashwanth C Francis; Gregory B Melikyan
Journal:  Viruses       Date:  2018-05-19       Impact factor: 5.048

Review 6.  Application of Advanced Light Microscopy to the Study of HIV and Its Interactions with the Host.

Authors:  Saveez Saffarian
Journal:  Viruses       Date:  2021-02-01       Impact factor: 5.048

7.  A New Generation of Functional Tagged Proteins for HIV Fluorescence Imaging.

Authors:  João I Mamede; Joseph Griffin; Stéphanie Gambut; Thomas J Hope
Journal:  Viruses       Date:  2021-02-28       Impact factor: 5.048

8.  Overlap Intensity: An ImageJ Macro for Analyzing the HIV-1 In Situ Uncoating Assay.

Authors:  Zachary Ingram; Hannah Matheney; Emma Wise; Courtney Weatherford; Amy E Hulme
Journal:  Viruses       Date:  2021-08-13       Impact factor: 5.048

9.  Differential CXCR4 expression on hematopoietic progenitor cells versus stem cells directs homing and engraftment.

Authors:  Sydney Felker; Archana Shrestha; Jeff Bailey; Devin M Pillis; Dylan Siniard; Punam Malik
Journal:  JCI Insight       Date:  2022-05-09
  9 in total

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