Imran Ahmad Khan1, Abdul Aziz1, Saira Bashir2, Muhammad Asif Raza3, Ghayoor Fatima4. 1. Department of Physiology and Pharmacology, Royal Institute Medical Science, Multan, Pakistan. 2. Department of Biochemistry, Royal Institute Medical Science, Multan, Pakistan. 3. Faculty of Veterinary Medicine, Higher College of Technology, Al Ain Men's College, UAE. 4. Organic Plant Production & Agroecosystems Research in the Tropics and Subtropics, University of Kassel, Germany.
Abstract
OBJECTIVE: To assess the counter-irritant and anti-inflammatory activity of Rumexvesicarius in dermatological use. METHODS: The animal-based experimental study was conducted at the Royal Institute of Medical Sciences, Multan, Pakistan, in November 2014. Sodium lauryl sulfate, phenol, histamine and sandpaper irritation models were used. Irritation was induced by the clockwise frictional movement of fine sandpaper to the ear of rabbits and then applying sodium lauryl sulfate, histamine and phenol single topical application onto the ear of the rabbit. The counter-irritant effect was determined by calculating the mean decrease in redness and erythema with those of control and standard dexamethasone. RESULTS: There were 20 rabbits in the study with a mean weight of 1.50±0.033 kg. R. vesicarius (100 and 150mg/mL) showed excellent counter-irritant effect when compared with control and standard groups. Both the doses depicted counter-irritant effect with the highest inhibition (94.42%) in sandpaper group, followed by sodium lauryl sulphate (90%), phenol (94.23%) and histamine (88.46%) irritation models respectively. CONCLUSIONS: Methanol leaf extract of R. vesicarius countered the effect of irritation in experimental animals. It showed significant effect in terms of dose and counter-irritancy time.
OBJECTIVE: To assess the counter-irritant and anti-inflammatory activity of Rumexvesicarius in dermatological use. METHODS: The animal-based experimental study was conducted at the Royal Institute of Medical Sciences, Multan, Pakistan, in November 2014. Sodium lauryl sulfate, phenol, histamine and sandpaper irritation models were used. Irritation was induced by the clockwise frictional movement of fine sandpaper to the ear of rabbits and then applying sodium lauryl sulfate, histamine and phenol single topical application onto the ear of the rabbit. The counter-irritant effect was determined by calculating the mean decrease in redness and erythema with those of control and standard dexamethasone. RESULTS: There were 20 rabbits in the study with a mean weight of 1.50±0.033 kg. R. vesicarius (100 and 150mg/mL) showed excellent counter-irritant effect when compared with control and standard groups. Both the doses depicted counter-irritant effect with the highest inhibition (94.42%) in sandpaper group, followed by sodium lauryl sulphate (90%), phenol (94.23%) and histamine (88.46%) irritation models respectively. CONCLUSIONS:Methanol leaf extract of R. vesicarius countered the effect of irritation in experimental animals. It showed significant effect in terms of dose and counter-irritancy time.
Authors: Md Mahmudul Hasan; Most Sayla Tasmin; Ahmed M El-Shehawi; Mona M Elseehy; Md Abu Reza; Ariful Haque Journal: BMC Complement Med Ther Date: 2021-09-04