Gwangil Kim1, Hee-Jung An1, Mi-Jung Lee2, Ji-Ye Song2, Ju-Yeon Jeong2, Ji-Hyun Lee3, Hye-Cheol Jeong4. 1. Department of Pathology, College of Medicine, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea. 2. Institute for Clinical Research, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea. 3. Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, CHA Bundang Medical Center, College of Medicine, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea. 4. Division of Respiratory and Critical Care Medicine, Department of Internal Medicine, CHA Bundang Medical Center, College of Medicine, CHA University, 59 Yatap-ro, Bundang-gu, Seongnam 463-712, South Korea. Electronic address: jhcmd@hanmail.net.
Abstract
OBJECTIVES: Cancer stem cells (CSCs) are considered to play a pivotal role in the process of invasion, metastasis and chemotherapy resistance. Diverse aberrantly expressed microRNAs (miRNAs) have been reported in lung cancer cells. However, there have been few reports about miRNAs that were associated with stemness and invasion of lung cancer. We investigated the role of miRNAs associated with characteristics of CSCs in non-small cell lung cancer (NSCLC). MATERIALS AND METHODS: We cultured A549 cells (lung adenocarcinoma) and HCC1588 cells (lung squamous cell carcinoma) in serum free media condition. We isolated sphere-forming NSCLC cells and examined the microRNA expression by microarray and qRT-PCT. By inhibition of CSC-associated microRNAs, we identified the changes of stemness and invasiveness in NSCLC. RESULTS AND CONCLUSION: We discovered 44 over-expressed, 42 down-regulated miRNAs in the sphere-forming cells compared with the parent cells of NSCLC. By in-silico database search, we selected miR-1246 and miR-1290 that were suspected to be associated with CSCs among aberrantly expressed miRNAs. Inhibition of miR-1246 and miR-1290 showed decreased stemness markers and epithelial-mesenchymal transition (EMT) markers in NSCLC. Anti-miR-1246 and anti-miR-1290 suppressed proliferation, sphere-formation, colony formation and invasion of NSCLC. CSCs-associated miR-1246, or miR-1290 may be important in the invasion or metastasis of NSCLC.
OBJECTIVES: Cancer stem cells (CSCs) are considered to play a pivotal role in the process of invasion, metastasis and chemotherapy resistance. Diverse aberrantly expressed microRNAs (miRNAs) have been reported in lung cancer cells. However, there have been few reports about miRNAs that were associated with stemness and invasion of lung cancer. We investigated the role of miRNAs associated with characteristics of CSCs in non-small cell lung cancer (NSCLC). MATERIALS AND METHODS: We cultured A549 cells (lung adenocarcinoma) and HCC1588 cells (lung squamous cell carcinoma) in serum free media condition. We isolated sphere-forming NSCLC cells and examined the microRNA expression by microarray and qRT-PCT. By inhibition of CSC-associated microRNAs, we identified the changes of stemness and invasiveness in NSCLC. RESULTS AND CONCLUSION: We discovered 44 over-expressed, 42 down-regulated miRNAs in the sphere-forming cells compared with the parent cells of NSCLC. By in-silico database search, we selected miR-1246 and miR-1290 that were suspected to be associated with CSCs among aberrantly expressed miRNAs. Inhibition of miR-1246 and miR-1290 showed decreased stemness markers and epithelial-mesenchymal transition (EMT) markers in NSCLC. Anti-miR-1246 and anti-miR-1290 suppressed proliferation, sphere-formation, colony formation and invasion of NSCLC. CSCs-associated miR-1246, or miR-1290 may be important in the invasion or metastasis of NSCLC.
Authors: Sherif Abdelhamed; John T Butler; Ben Doron; Amber Halse; Eneida Nemecek; Phillip A Wilmarth; Daniel L Marks; Bill H Chang; Terzah Horton; Peter Kurre Journal: EMBO Rep Date: 2019-06-17 Impact factor: 8.807
Authors: L Moyal; C Arkin; B Gorovitz-Haris; C Querfeld; S Rosen; J Knaneh; I Amitay-Laish; H Prag-Naveh; J Jacob-Hirsch; E Hodak Journal: Br J Dermatol Date: 2021-08-13 Impact factor: 11.113