Pawan Kumar Maurya1,2, Prabhanshu Kumar2, Pranjal Chandra3. 1. a Interdisciplinary Laboratory for Clinical Neuroscience (LiNC) , Department of Psychiatry, Universidade Federal de Sao Paulo - UNIFESP , Sao Paulo , Brazil . 2. b Amity Institute of Biotechnology , Amity University Uttar Pradesh , Noida, Uttar Pradesh , India , and. 3. c Department of Biosciences and Bioengineering , Indian Institute of Technology - Guwahati , Guwahati , Assam , India.
Abstract
CONTEXT: Glucose-6-phosphate dehydrogenase (G6PD) is an important enzyme of hexose monophosphate shunt, involved in the biosynthesis of reduced nicotinamide adenine dinucleotide phosphate hydrogen (NADPH). OBJECTIVE: This study was designed to investigate age-dependent changes in human erythrocyte G6PD activity. The G6PD activity pattern was correlated with reduced glutathione (GSH) and total antioxidant potential in terms of FRAP (ferric reducing ability of plasma) value. MATERIALS AND METHODS: We analyzed normal, healthy subjects of both sexes between the ages of 20 and 80 years. G6PD activity was determined by Burties method. RESULTS: We observe a significant age-dependent decrease in G6PD activity (p < 0.0001). It was positively correlated with GSH (r = 0.5706) and total antioxidant potential (r = 0.7723) as a function of human age. DISCUSSION AND CONCLUSION: Our findings on erythrocyte G6PD and their correlation with GSH and FRAP provide evidence of a higher oxidative stress in old age population.
CONTEXT: Glucose-6-phosphate dehydrogenase (G6PD) is an important enzyme of hexose monophosphate shunt, involved in the biosynthesis of reduced nicotinamide adenine dinucleotide phosphate hydrogen (NADPH). OBJECTIVE: This study was designed to investigate age-dependent changes in human erythrocyte G6PD activity. The G6PD activity pattern was correlated with reduced glutathione (GSH) and total antioxidant potential in terms of FRAP (ferric reducing ability of plasma) value. MATERIALS AND METHODS: We analyzed normal, healthy subjects of both sexes between the ages of 20 and 80 years. G6PD activity was determined by Burties method. RESULTS: We observe a significant age-dependent decrease in G6PD activity (p < 0.0001). It was positively correlated with GSH (r = 0.5706) and total antioxidant potential (r = 0.7723) as a function of human age. DISCUSSION AND CONCLUSION: Our findings on erythrocyte G6PD and their correlation with GSH and FRAP provide evidence of a higher oxidative stress in old age population.
Authors: Patrik Krumpolec; Radka Klepochová; Ivica Just; Marjeta Tušek Jelenc; Ivan Frollo; Jozef Ukropec; Barbara Ukropcová; Siegfried Trattnig; Martin Krššák; Ladislav Valkovič Journal: Front Physiol Date: 2020-06-29 Impact factor: 4.566
Authors: Angelo D'Alessandro; Xiaoyun Fu; Tamir Kanias; Julie A Reisz; Rachel Culp-Hill; Yuelong Guo; Mark T Gladwin; Grier Page; Steven Kleinman; Marion Lanteri; Mars Stone; Michael P Busch; James C Zimring Journal: Haematologica Date: 2021-05-01 Impact factor: 9.941