Literature DB >> 2671055

Improved method for immunoperoxidase detection of membrane antigens in frozen sections.

M A Green1, L Sviland, A J Malcolm, A D Pearson.   

Abstract

The visualisation of membrane antigens in frozen sections using metal-enhanced 3,3'diaminobenzidine (DAB) was investigated. Particular attention was paid to the degree of reaction enhancement, the disruptive effect on morphological detail, and the ease of the techniques. The best result was obtained using nickel-modified DAB at pH 6.0 to develop the peroxidase enzyme, with further enhancement in cobalt chloride at neutral pH. Silver methenamine enhancement is also possible but can give rise to non-specific or background staining.

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Year:  1989        PMID： 2671055      PMCID： PMC1142069          DOI： 10.1136/jcp.42.8.875

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  9 in total

1.  Increased sensitivity in peroxidase immunocytochemistry. A comparative study of a number of peroxidase visualization methods employing a model system.

Authors:  L Scopsi; L I Larsson
Journal:  Histochemistry       Date:  1986

2.  Intensification of 3,3'-diaminobenzidine precipitation using the ferric ferricyanide reaction, and its application in the double-immunoperoxidase technique.

Authors:  Z Nemes
Journal:  Histochemistry       Date:  1987

3.  PAP labeling enhancement by osmium tetroxide-potassium ferrocyanide treatment.

Authors:  E F Lascano; M I Berria
Journal:  J Histochem Cytochem       Date:  1988-06       Impact factor: 2.479

4.  Visualization of peptide-immunoreactive processes on serotonin-immunoreactive cells using two-color immunoperoxidase staining.

Authors:  M B Hancock
Journal:  J Histochem Cytochem       Date:  1984-03       Impact factor: 2.479

5.  Imidazole increases the sensitivity of the cytochemical reaction for peroxidase with diaminobenzidine at a neutral pH.

Authors:  W Straus
Journal:  J Histochem Cytochem       Date:  1982-05       Impact factor: 2.479

6.  High-grade intensification of the end-product of the diaminobenzidine reaction for peroxidase histochemistry.

Authors:  F Gallyas; T Görcs; I Merchenthaler
Journal:  J Histochem Cytochem       Date:  1982-02       Impact factor: 2.479

7.  Facilitated light microscopic cytochemical diagnosis of acute myelogenous leukemia.

Authors:  J S Hanker; W W Ambrose; C J James; J Mandelkorn; P E Yates; S A Gall; E H Bossen; J W Fay; J Laszlo; J O Moore
Journal:  Cancer Res       Date:  1979-05       Impact factor: 12.701

8.  Color modification of diaminobenzidine (DAB) precipitation by metallic ions and its application for double immunohistochemistry.

Authors:  S M Hsu; E Soban
Journal:  J Histochem Cytochem       Date:  1982-10       Impact factor: 2.479

9.  Standardization of various applications of methacrylate embedding and silver methenamine for light and electron microscopy immunocytochemistry.

Authors:  E M Rodríguez; R Yulis; B Peruzzo; G Alvial; R Andrade
Journal:  Histochemistry       Date:  1984
  9 in total
  10 in total

1.  Permissive cytomegalovirus infection of primary villous term and first trimester trophoblasts.

Authors:  D G Hemmings; R Kilani; C Nykiforuk; J Preiksaitis; L J Guilbert
Journal:  J Virol       Date:  1998-06       Impact factor: 5.103

2.  Diagnostic features of transfusion associated graft versus host disease.

Authors:  A L Appleton; L Sviland; A D Pearson; J Wilkes; M A Green; A J Malcolm
Journal:  J Clin Pathol       Date:  1994-06       Impact factor: 3.411

3.  Expression of the bcl-2 oncogene protein is not specific for the 14;18 chromosomal translocation.

Authors:  F Pezzella; A G Tse; J L Cordell; K A Pulford; K C Gatter; D Y Mason
Journal:  Am J Pathol       Date:  1990-08       Impact factor: 4.307

4.  Carcinoma-associated monoclonal antibodies in head and neck carcinoma. Immunohistochemistry and biodistribution of monoclonal antibodies 175F4 and 175F11.

Authors:  A J Balm; P C Hageman; C L Mulder; J Hilkens
Journal:  Eur Arch Otorhinolaryngol       Date:  1992       Impact factor: 2.503

5.  A high iodine intake in Wistar rats results in the development of a thyroid-associated ectopic thymic tissue and is accompanied by a low thyroid autoimmune reactivity.

Authors:  P Mooij; H J de Wit; H A Drexhage
Journal:  Immunology       Date:  1994-02       Impact factor: 7.397

6.  Sensitive detection of human growth hormone mRNA in routinely formalin-fixed, paraffin-embedded transgenic mouse tissues by non-isotopic in situ hybridization.

Authors:  J Ehrlein; R Wanke; S Weis; G Brem; W Hermanns
Journal:  Histochemistry       Date:  1994-08

7.  Integrin alpha 6/beta 4 complex is located in hemidesmosomes, suggesting a major role in epidermal cell-basement membrane adhesion.

Authors:  A Sonnenberg; J Calafat; H Janssen; H Daams; L M van der Raaij-Helmer; R Falcioni; S J Kennel; J D Aplin; J Baker; M Loizidou
Journal:  J Cell Biol       Date:  1991-05       Impact factor: 10.539

8.  Automatic quantification of microvessel density in urinary bladder carcinoma.

Authors:  K Wester; P Ranefall; E Bengtsson; C Busch; P U Malmström
Journal:  Br J Cancer       Date:  1999-12       Impact factor: 7.640

9.  A simple, inexpensive, robust and sensitive dot-blot assay for equal detection of the nonstructural-1 glycoprotein of all dengue virus serotypes.

Authors:  Andrew K I Falconar; Claudia M E Romero-Vivas
Journal:  Virol J       Date:  2013-04-22       Impact factor: 4.099

10.  In situ strategy for biomedical target localization via nanogold nucleation and secondary growth.

Authors:  Akira Sawaguchi; Takeshi Kamimura; Nobuyasu Takahashi; Atsushi Yamashita; Yujiro Asada; Hiroyuki Imazato; Fumiyo Aoyama; Akiko Wakui; Takeshi Sato; Narantsog Choijookhuu; Yoshitaka Hishikawa
Journal:  Commun Biol       Date:  2021-06-10
  10 in total

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