Literature DB >> 2671036

Method for improved extraction of DNA from Nocardia asteroides.

M J Loeffelholz1, D R Scholl.   

Abstract

In a variation of standard DNA extraction methods, Nocardia asteroides was repeatedly exposed to sodium dodecyl sulfate at 60 degrees C for 30 min; each extraction was followed by centrifugation, removal of the nucleic acid-rich supernatant, and suspension of the cell pellet in fresh sodium dodecyl sulfate. The pooled supernatants contained a substantially higher amount of DNA than the first supernatant alone. The possible implications of this procedure on the development of DNA probes are discussed.

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Year:  1989        PMID： 2671036      PMCID： PMC267689          DOI： 10.1128/jcm.27.8.1880-1881.1989

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  5 in total

1.  Evaluation of a commercial gene probe for identification of Legionella cultures.

Authors:  H W Wilkinson; J S Sampson; B B Plikaytis
Journal:  J Clin Microbiol       Date:  1986-02       Impact factor: 5.948

2.  Comparison of Gen-Probe commercial kit and culture technique for the diagnosis of Mycoplasma pneumoniae infection.

Authors:  R Dular; R Kajioka; S Kasatiya
Journal:  J Clin Microbiol       Date:  1988-05       Impact factor: 5.948

3.  Rapid identification of Mycobacterium avium complex in culture using DNA probes.

Authors:  T A Drake; J A Hindler; O G Berlin; D A Bruckner
Journal:  J Clin Microbiol       Date:  1987-08       Impact factor: 5.948

4.  Deoxyribonucleic acid reassociation in the classification of the 'rhodochrous' complex and allied taxa.

Authors:  M Mordarski; K Szyba; G Pulverer; M Goodfellow
Journal:  J Gen Microbiol       Date:  1976-06

5.  No correlation exists between the conjugative transfer of the autotrophic character and that of plasmids in Nocardia opaca strains.

Authors:  C Sensfuss; M Reh; H G Schlegel
Journal:  J Gen Microbiol       Date:  1986-04
  5 in total
  8 in total

1.  A rapid and gentle method for isolation of genomic DNA from pathogenic Nocardia spp.

Authors:  R D Torres; C A Oletta; H Zlotnik
Journal:  Clin Diagn Lab Immunol       Date:  1996-09

2.  Multiple copies of the 16S rRNA gene in Nocardia nova isolates and implications for sequence-based identification procedures.

Authors:  Patricia S Conville; Frank G Witebsky
Journal:  J Clin Microbiol       Date:  2005-06       Impact factor: 5.948

3.  Analysis of multiple differing copies of the 16S rRNA gene in five clinical isolates and three type strains of Nocardia species and implications for species assignment.

Authors:  Patricia S Conville; Frank G Witebsky
Journal:  J Clin Microbiol       Date:  2007-02-14       Impact factor: 5.948

4.  Metabolic pathway for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) formation in Nocardia corallina: inactivation of mutB by chromosomal integration of a kanamycin resistance gene.

Authors:  H F Valentin; D Dennis
Journal:  Appl Environ Microbiol       Date:  1996-02       Impact factor: 4.792

5.  Nocardia veterana as a pathogen in North American patients.

Authors:  Patricia S Conville; June M Brown; Arnold G Steigerwalt; Judy W Lee; Dorothy E Byrer; Victoria L Anderson; Susan E Dorman; Steven M Holland; Barbara Cahill; Karen C Carroll; Frank G Witebsky
Journal:  J Clin Microbiol       Date:  2003-06       Impact factor: 5.948

6.  Nocardia wallacei sp. nov. and Nocardia blacklockiae sp. nov., human pathogens and members of the "Nocardia transvalensis Complex".

Authors:  Patricia S Conville; June M Brown; Arnold G Steigerwalt; Barbara A Brown-Elliott; Frank G Witebsky
Journal:  J Clin Microbiol       Date:  2008-02-06       Impact factor: 5.948

7.  Mycobacterium chelonae-abscessus complex associated with sinopulmonary disease, Northeastern USA.

Authors:  Keith E Simmon; Barbara A Brown-Elliott; Perry G Ridge; Jacob D Durtschi; Linda Bridge Mann; E Susan Slechta; Arnold G Steigerwalt; Benjamin D Moser; Anne M Whitney; June M Brown; Karl V Voelkerding; Karin L McGowan; Anne F Reilly; Thomas J Kirn; W Ray Butler; Paul H Edelstein; Richard J Wallace; Cathy A Petti
Journal:  Emerg Infect Dis       Date:  2011-09       Impact factor: 6.883

8.  DNA extraction from nocardia species for special genes analysis using PCR.

Authors:  Mehdi Fatahi Bafghi; Seyyed Saeed Eshraghi; Parvin Heidarieh; Shadi Habibnia; Masoumeh Rasouli Nasab
Journal:  N Am J Med Sci       Date:  2014-05
  8 in total

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