Mustafa Aykut Ozpur1, Ethem Guneren, Halil Ibrahim Canter, Mehmet Veli Karaaltin, Ercument Ovali, Fatma Nilay Yogun, Emre Gonenc Baygol, Suleyman Kaplan. 1. Adiyaman, Istanbul, Kirklareli, and Samsun, Turkey From the Department of Plastic, Reconstructive and Aesthetic Surgery, Government Hospital; the Department of Plastic, Reconstructive and Aesthetic Surgery, Bezmialem Vakif University; the Department of Plastic, Reconstructive and Aesthetic Surgery, Acibadem University; the Department of Haematolgoy, Acibadem University; the Department of Plastic, Reconstructive and Aesthetic Surgery, Government Hospital; and the Department of Histology, Ondokuz Mayis University.
Abstract
BACKGROUND: The aim of this study was to generate skin tissue using adipose tissue-derived mesenchymal stem cells. METHODS: Thirty Wistar albino rats were used. A 2-cm-diameter full-thickness skin defect on the back of each rat was formed. A secondary wound healing model was constituted in group 1, fibrin matrix only was applied in group 2, a keratinocyte-coated fibrin matrix was applied in group 3, an adipose tissue-derived mesenchymal stem cell-seeded fibrin matrix was applied in group 4, and a keratinocyte-coated and adipose tissue-derived mesenchymal stem cell-seeded fibrin matrix was applied to the defects in group 5. RESULTS: A similar form of wound healing, with contraction from the edges and ulceration at the center, was observed in groups 1, 2, and 3. The wound contraction was reduced in group 4, and the epidermis was creeping from the surrounding tissue but with some ulcerations in the central part of the wounds. In group 5, the defect area was almost totally epithelialized, with minimal wound contraction. By microscopic analysis, significant increases in the collagen volume ratios and vascular volume ratios were determined in groups 4 and 5. From the fluorescent micrographs, fibroblastic differentiation and extracellular matrix synthesis, endothelial differentiation of stem cells, and neoangiogenesis and epithelium derived from marked keratinocytes were observed in group 5. CONCLUSION: Formation of the adipose tissue-derived mesenchymal stem cell-seeded and keratinocyte-coated autologous fibrin scaffold leads to significant skin replacement.
BACKGROUND: The aim of this study was to generate skin tissue using adipose tissue-derived mesenchymal stem cells. METHODS: Thirty Wistar albino rats were used. A 2-cm-diameter full-thickness skin defect on the back of each rat was formed. A secondary wound healing model was constituted in group 1, fibrin matrix only was applied in group 2, a keratinocyte-coated fibrin matrix was applied in group 3, an adipose tissue-derived mesenchymal stem cell-seeded fibrin matrix was applied in group 4, and a keratinocyte-coated and adipose tissue-derived mesenchymal stem cell-seeded fibrin matrix was applied to the defects in group 5. RESULTS: A similar form of wound healing, with contraction from the edges and ulceration at the center, was observed in groups 1, 2, and 3. The wound contraction was reduced in group 4, and the epidermis was creeping from the surrounding tissue but with some ulcerations in the central part of the wounds. In group 5, the defect area was almost totally epithelialized, with minimal wound contraction. By microscopic analysis, significant increases in the collagen volume ratios and vascular volume ratios were determined in groups 4 and 5. From the fluorescent micrographs, fibroblastic differentiation and extracellular matrix synthesis, endothelial differentiation of stem cells, and neoangiogenesis and epithelium derived from marked keratinocytes were observed in group 5. CONCLUSION: Formation of the adipose tissue-derived mesenchymal stem cell-seeded and keratinocyte-coated autologous fibrin scaffold leads to significant skin replacement.
Authors: Rita Businaro; Mariangela Corsi; Tania Di Raimo; Sergio Marasco; Debra L Laskin; Bruno Salvati; Raffaele Capoano; Serafino Ricci; Camilla Siciliano; Giacomo Frati; Elena De Falco Journal: Ann N Y Acad Sci Date: 2016-07-19 Impact factor: 5.691
Authors: Shirin Nour; Nafiseh Baheiraei; Rana Imani; Mohammad Khodaei; Akram Alizadeh; Navid Rabiee; S Mohammad Moazzeni Journal: J Mater Sci Mater Med Date: 2019-10-19 Impact factor: 3.896
Authors: Biraja C Dash; Zhenzhen Xu; Lawrence Lin; Andrew Koo; Sifon Ndon; Francois Berthiaume; Alan Dardik; Henry Hsia Journal: Bioengineering (Basel) Date: 2018-03-09