Sebastian Rauschert1, Olaf Uhl1, Berthold Koletzko1, Franca Kirchberg1, Trevor A Mori1, Rae-Chi Huang1, Lawrence J Beilin1, Christian Hellmuth1, Wendy H Oddy1. 1. Division of Metabolic and Nutritional Medicine (S.R., O.U., B.K., F.K., C.H.), Dr von Hauner Children's Hospital, Ludwig Maximilians University of Munich, D-80337 Munich, Germany; Royal Perth Hospital Unit (T.M., L.J.B.), School of Medicine and Pharmacology, The University of Western Australia, 6000 Perth, Western Australia, Australia; and Telethon Kids Institute (R.-C.H., W.H.O.), The University of Western Australia, 6009 Perth, Western Australia, Australia.
Abstract
CONTEXT: Obesity and related diseases have become a global public health burden. Identifying biomarkers will lead to a better understanding of the underlying mechanisms associated with obesity and the pathways leading to insulin resistance (IR) and diabetes. OBJECTIVE: This study aimed to identify the lipidomic biomarkers associated with obesity and IR using plasma samples from a population-based cohort of young adults. DESIGN AND SETTING: The Western Australian Pregnancy Cohort (Raine) study enrolled 2900 pregnant women from 1989 to 1991. The 20-year follow-up was conducted between March 2010 and April 2012. Participants and Samples: Plasma samples from 1176 subjects aged 20 years were analyzed using mass spectrometry-based metabolomics. MAIN OUTCOME MEASURES: Associations of analytes with markers of obesity and IR including body mass index, waist circumference, homeostasis model assessment (HOMA-IR), and insulin were examined. Analyses were stratified by body mass index and adjusted for lifestyle and other factors. RESULTS: Waist circumference was positively associated with seven sphingomyelins and five diacylphosphatidylcholines and negatively associated with two lysophosphatidylcholines. HOMA-IR was negatively associated with two diacylphosphatidylcholines and positively with one lysophosphatidylcholine and one diacylphosphatidylcholine. No significant association was found in the obese/overweight group of the HOMA-IR model. In the normal-weight group, one lysophosphatidylcholine was increased. CONCLUSION: A possible discriminative effect of sphingomyelins, particularly those with two double bonds, and lysophosphatidylcholines was identified between subjects with normal weight and obesity independent of low-density lipoprotein cholesterol and high-density lipoprotein cholesterol concentrations. Our results suggest weight status-dependent mechanisms for the development of IR with lysophosphatidylcholine C14:0 as a key metabolite in nonobese IR.
CONTEXT: Obesity and related diseases have become a global public health burden. Identifying biomarkers will lead to a better understanding of the underlying mechanisms associated with obesity and the pathways leading to insulin resistance (IR) and diabetes. OBJECTIVE: This study aimed to identify the lipidomic biomarkers associated with obesity and IR using plasma samples from a population-based cohort of young adults. DESIGN AND SETTING: The Western Australian Pregnancy Cohort (Raine) study enrolled 2900 pregnant women from 1989 to 1991. The 20-year follow-up was conducted between March 2010 and April 2012. Participants and Samples: Plasma samples from 1176 subjects aged 20 years were analyzed using mass spectrometry-based metabolomics. MAIN OUTCOME MEASURES: Associations of analytes with markers of obesity and IR including body mass index, waist circumference, homeostasis model assessment (HOMA-IR), and insulin were examined. Analyses were stratified by body mass index and adjusted for lifestyle and other factors. RESULTS: Waist circumference was positively associated with seven sphingomyelins and five diacylphosphatidylcholines and negatively associated with two lysophosphatidylcholines. HOMA-IR was negatively associated with two diacylphosphatidylcholines and positively with one lysophosphatidylcholine and one diacylphosphatidylcholine. No significant association was found in the obese/overweight group of the HOMA-IR model. In the normal-weight group, one lysophosphatidylcholine was increased. CONCLUSION: A possible discriminative effect of sphingomyelins, particularly those with two double bonds, and lysophosphatidylcholines was identified between subjects with normal weight and obesity independent of low-density lipoprotein cholesterol and high-density lipoprotein cholesterol concentrations. Our results suggest weight status-dependent mechanisms for the development of IR with lysophosphatidylcholine C14:0 as a key metabolite in nonobese IR.
Authors: Christopher Papandreou; Mònica Bulló; Miguel Ruiz-Canela; Courtney Dennis; Amy Deik; Daniel Wang; Marta Guasch-Ferré; Edward Yu; Cristina Razquin; Dolores Corella; Ramon Estruch; Emilio Ros; Montserrat Fitó; Miquel Fiol; Liming Liang; Pablo Hernández-Alonso; Clary B Clish; Miguel A Martínez-González; Frank B Hu; Jordi Salas-Salvadó Journal: Am J Clin Nutr Date: 2019-03-01 Impact factor: 7.045
Authors: Christian Hellmuth; Olaf Uhl; Marie Standl; Hans Demmelmair; Joachim Heinrich; Berthold Koletzko; Elisabeth Thiering Journal: Obes Facts Date: 2017-04-05 Impact factor: 3.942