Daniela Valadão Rosa1, Vitor Bortolo Rezende2, Bruno Silva Costa3, Fernando Mudado4, Manuel Schütze5, Karen Cecília Torres6, Luíza Conceição Martins7, Carlos Alberto Moreira-Filho8, Debora Marques Miranda9, Marco Aurélio Romano-Silva10. 1. INCT de Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Alfredo Balena, 190, Belo Horizonte, MG, Brazil. Electronic address: danielavaladao@gmail.com. 2. INCT de Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Alfredo Balena, 190, Belo Horizonte, MG, Brazil. Electronic address: rezendevb@gmail.com. 3. Centre for Neurology and Neurosurgery at Santa Casa de Misericórdia, Belo Horizonte, MG, Brazil. Electronic address: costabs@gmail.com. 4. INCT de Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Alfredo Balena, 190, Belo Horizonte, MG, Brazil. Electronic address: fernandomudado@gmail.com. 5. INCT de Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Alfredo Balena, 190, Belo Horizonte, MG, Brazil. Electronic address: mschutze@gmail.com. 6. INCT de Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Alfredo Balena, 190, Belo Horizonte, MG, Brazil. Electronic address: kcltorres@gmail.com. 7. INCT de Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Alfredo Balena, 190, Belo Horizonte, MG, Brazil. Electronic address: luizamart@gmail.com. 8. Department of Pediatrics, Faculdade de Medicina da Universidade de São Paulo, São Paulo, SP, Brazil. Electronic address: carlos.moreira@hc.fm.usp.br. 9. INCT de Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Alfredo Balena, 190, Belo Horizonte, MG, Brazil; Department of Pediatrics, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. Electronic address: debora.m.miranda@gmail.com. 10. INCT de Medicina Molecular, Faculdade de Medicina, Universidade Federal de Minas Gerais, Av Alfredo Balena, 190, Belo Horizonte, MG, Brazil. Electronic address: romano-silva@ufmg.br.
Abstract
OBJECTIVE: To compare the proinflammatory and anti-inflammatory cytokine expression profile of CD4(+) and CD8(+) T lymphocytes between drug resistant mesial Temporal Lobe Epilepsy (mTLE) patients and healthy subjects. METHODS: mTLE patients were enrolled at the Neurology Center of Santa Casa de Misericórdia de Belo Horizonte (SCM-BH) and healthy volunteers were selected at Universidade Federal de Minas Gerais. Individuals from both groups accepted to participate in this study and signed an informed consent. Peripheral venous blood samples were collected using sodium heparin vacuum tubes on the day before the surgery and in the interictal period, isolated from whole blood using Ficoll/Hypaque followed by flow cytometry analysis. Data analysis was performed using FlowJo. RESULTS: Compared to healthy individuals, mTLE patients showed reduced frequency of CD8(+) T lymphocytes expressing IFN-γ, TNF-α, IL-17 and IL-4. Moreover, mTLE patients presented increased frequency of CD4(+) T lymphocytes expressing IL-6 when compared to healthy volunteers. DISCUSSION: Epilepsy is the third most common chronic brain disorder. Mesial temporal lobe epilepsy (mTLE) is a major and severe form of epilepsy and 30% of the mTLE patients do not respond to conventional medications. Our data suggest that mTLE patients have distinct immunological profiles that are related to disease pathophysiology.
OBJECTIVE: To compare the proinflammatory and anti-inflammatory cytokine expression profile of CD4(+) and CD8(+) T lymphocytes between drug resistant mesial Temporal Lobe Epilepsy (mTLE) patients and healthy subjects. METHODS: mTLE patients were enrolled at the Neurology Center of Santa Casa de Misericórdia de Belo Horizonte (SCM-BH) and healthy volunteers were selected at Universidade Federal de Minas Gerais. Individuals from both groups accepted to participate in this study and signed an informed consent. Peripheral venous blood samples were collected using sodium heparin vacuum tubes on the day before the surgery and in the interictal period, isolated from whole blood using Ficoll/Hypaque followed by flow cytometry analysis. Data analysis was performed using FlowJo. RESULTS: Compared to healthy individuals, mTLE patients showed reduced frequency of CD8(+) T lymphocytes expressing IFN-γ, TNF-α, IL-17 and IL-4. Moreover, mTLE patients presented increased frequency of CD4(+) T lymphocytes expressing IL-6 when compared to healthy volunteers. DISCUSSION: Epilepsy is the third most common chronic brain disorder. Mesial temporal lobe epilepsy (mTLE) is a major and severe form of epilepsy and 30% of the mTLE patients do not respond to conventional medications. Our data suggest that mTLE patients have distinct immunological profiles that are related to disease pathophysiology.