Cry1ab/c in different stages of growth in transgenic rice Bt-shanyou63.

The relationship between the mRNA level and the corresponding protein level of the cry1Ab/c gene is not well characterized in transgenic rice (Bt-ShanYou63). In this study, we compared cry1Ab/c mRNA and its protein expression in leaves at different growth stages in Bt-ShanYou63 rice. The results demonstrated that both cry1Ab/c mRNA and its protein levels changed at all of the growth stages. The cry1Ab/c transcript levels in the leaves were highest during the grain filling stage (3.29, cry/actin) and lowest during the seeding stage (1.06, cry/actin), and the protein levels of Cry1Ab/c was also highest at the grain filling stage (5.71 microgram x g-1 fresh weight, fw) and lowest during the seeding stage (2.08 microgram x g(-1) fw). There was a significant correlation between cry1Ab/c mRNA levels and the protein concentrations (r=0.742, p < 0.01). However, a linear relationship was not observed between cry1Ab/c mRNA levels and the protein levels, and the trend for mRNA expression levels was not consistent with the Cry1Ab/c protein levels in the same growth period in Bt-ShanYou63 rice.