Roberto Monastero1,2, Sara García-Serrano3,4, Ana Lago-Sampedro1,2, Francisca Rodríguez-Pacheco1,2, Natalia Colomo1,2, Sonsoles Morcillo5,6, Gracia M Martín-Nuñez1,2, Juan M Gomez-Zumaquero7, Eduardo García-Fuentes1,5, Federico Soriguer1,2, Gemma Rojo-Martínez1,2, Eva García-Escobar8,9. 1. UGC Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Regional de Málaga, Málaga, Spain. 2. CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM CB07/08/0019), Instituto de Salud Carlos III, Málaga, Spain. 3. UGC Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Regional de Málaga, Málaga, Spain. garciasara79@hotmail.com. 4. CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM CB07/08/0019), Instituto de Salud Carlos III, Málaga, Spain. garciasara79@hotmail.com. 5. CIBER de Obesidad y Nutrición (CIBEROBN CB06/03/0018), Instituto de Salud Carlos III, Málaga, Spain. 6. UGC Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Virgen de la Victoria, Málaga, Spain. 7. ECAI de Genomica del Instituto de Investigación Biomédica de Málaga (IBIMA), Málaga, Spain. 8. UGC Endocrinología y Nutrición, Instituto de Investigación Biomédica de Málaga (IBIMA), Hospital Universitario Regional de Málaga, Málaga, Spain. eyring@gmail.com. 9. CIBER de Diabetes y Enfermedades Metabólicas asociadas (CIBERDEM CB07/08/0019), Instituto de Salud Carlos III, Málaga, Spain. eyring@gmail.com.
Abstract
PURPOSE: We have investigated the epigenetic regulation by dietary fatty acids of Vegfb levels in rats' white adipose tissue and 3T3-L1 cells. METHODS: A group of rats were assigned to three diets, each one with a different composition of saturated, monounsaturated and polyunsaturated fatty acids. Samples of white adipose tissues were taken for the methylation and expression studies. Additionally, 3T3-L1 cells were treated with palmitic, oleic, and linoleic fatty acids. After treatment, cells were harvested and genetic material was extracted for the analysis of Vegfb levels. RESULTS: We report evidence of changes in the methylation levels of the CpG island at the Vegfb promoter and in the Vegfb expression levels in vivo and in vitro by dietary fatty acid, with the main contribution of the linoleic fatty acid. Vegfb promoter methylation levels were closely related to the Vegfb gene expression. CONCLUSION: According to our results, the regulation of Vegfb gene expression by dietary fatty acids may be mediated, at least in part, by epigenetic modifications on Vegfb promoter methylation. Considering the deep association between angiogenesis and tissue growth, we suggest the nutriepigenetic regulation of Vegfb as a key target in the control of the adipose tissue expansion.
PURPOSE: We have investigated the epigenetic regulation by dietary fatty acids of Vegfb levels in rats' white adipose tissue and 3T3-L1 cells. METHODS: A group of rats were assigned to three diets, each one with a different composition of saturated, monounsaturated and polyunsaturated fatty acids. Samples of white adipose tissues were taken for the methylation and expression studies. Additionally, 3T3-L1 cells were treated with palmitic, oleic, and linoleic fatty acids. After treatment, cells were harvested and genetic material was extracted for the analysis of Vegfb levels. RESULTS: We report evidence of changes in the methylation levels of the CpG island at the Vegfb promoter and in the Vegfb expression levels in vivo and in vitro by dietary fatty acid, with the main contribution of the linoleic fatty acid. Vegfb promoter methylation levels were closely related to the Vegfb gene expression. CONCLUSION: According to our results, the regulation of Vegfb gene expression by dietary fatty acids may be mediated, at least in part, by epigenetic modifications on Vegfb promoter methylation. Considering the deep association between angiogenesis and tissue growth, we suggest the nutriepigenetic regulation of Vegfb as a key target in the control of the adipose tissue expansion.
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