AIMS: Ameloblastoma AME is a benign tumour characterized by local invasiveness, high recurrence rates, and diverse histological patterns. The oxygen concentration is reduced in specific areas of the tumour microenvironment, which leads to intratumoral hypoxia. Crosstalk between NOTCH1, a disintegrin and metalloproteinase 12 (ADAM-12), hypoxia-inducible factor 1α (HIF-1α) and heparin-binding epidermal growth factor (HB-EGF) under hypoxic conditions has been implicated in invadopodia formation, tumour invasiveness, and metastasis development. The aim of this study was to analyse the expression of these proteins, in order to further elucidate the mechanisms underlying AME invasiveness. METHODS AND RESULTS: Twenty cases of AME, eight calcifying cystic odontogenic tumours CCOTs and 10 samples of dental follicle were used to investigate the expression of these proteins by immunohistochemistry with the primary antibodies anti-NOTCH1, anti-ADAM-12, anti-HIF-1α, and anti-HB-EGF. Immunostaining results were expressed as the percentage of stained area in images acquired in an AxioScope microscope equipped with an AxioCamHRc camera and a × 40 objective. The results showed that immunoexpression of all proteins was higher in the AME samples than in the CCOT and dental follicle samples (P < 0.05). CONCLUSIONS: AME showed an increased presence of proteins associated with tumour invasiveness, which indicates a possible role of these proteins in the biological behaviour of this tumour.
AIMS: Ameloblastoma AME is a benign tumour characterized by local invasiveness, high recurrence rates, and diverse histological patterns. The oxygen concentration is reduced in specific areas of the tumour microenvironment, which leads to intratumoral hypoxia. Crosstalk between NOTCH1, a disintegrin and metalloproteinase 12 (ADAM-12), hypoxia-inducible factor 1α (HIF-1α) and heparin-binding epidermal growth factor (HB-EGF) under hypoxic conditions has been implicated in invadopodia formation, tumour invasiveness, and metastasis development. The aim of this study was to analyse the expression of these proteins, in order to further elucidate the mechanisms underlying AME invasiveness. METHODS AND RESULTS: Twenty cases of AME, eight calcifying cystic odontogenic tumours CCOTs and 10 samples of dental follicle were used to investigate the expression of these proteins by immunohistochemistry with the primary antibodies anti-NOTCH1, anti-ADAM-12, anti-HIF-1α, and anti-HB-EGF. Immunostaining results were expressed as the percentage of stained area in images acquired in an AxioScope microscope equipped with an AxioCamHRc camera and a × 40 objective. The results showed that immunoexpression of all proteins was higher in the AME samples than in the CCOT and dental follicle samples (P < 0.05). CONCLUSIONS: AME showed an increased presence of proteins associated with tumour invasiveness, which indicates a possible role of these proteins in the biological behaviour of this tumour.
Authors: Natacha M M da Costa; Adriane S de Siqueira; André L R Ribeiro; Maria S da Silva Kataoka; Ruy G Jaeger; Sérgio M de Alves-Júnior; Andrew M Smith; João de Jesus Viana Pinheiro Journal: Clin Oral Investig Date: 2017-02-25 Impact factor: 3.573
Authors: Natacha Malu Miranda da Costa; Caio Tadashi Saab Abe; Geovanni Pereira Mitre; Ricardo Alves Mesquita; Maria Sueli da Silva Kataoka; André Luis Ribeiro Ribeiro; Ruy Gastaldoni Jaeger; Sérgio de Melo Alves-Júnior; Andrew Mark Smith; João de Jesus Viana Pinheiro Journal: Cells Date: 2019-07-17 Impact factor: 6.600
Authors: Katherine Julissa Palma Valladares; Karolyny Martins Balbinot; Antonia Taiane Lopes de Moraes; Maria Sueli da Silva Kataoka; Aline Maria Pereira Cruz Ramos; Rommel Thiago Jucá Ramos; Artur Luiz da Costa da Silva; Ricardo Alves Mesquita; David Normando; Sérgio de Melo Alves Júnior; João de Jesus Viana Pinheiro Journal: Int J Dent Date: 2021-12-27