Hongliang Zhao1, Yan Chen2, Cuiping Zhang3, Xiaobing Fu4. 1. Burns and Plastic Department, Miyun County Hospital of Capital Medical University, 383#, Yangguang Road, Miyun County, Beijing 101500, China. 2. Wounds Repair and Tissue Regeneration Laboratory, The First Affiliated Hospital, General Hospital of PLA, 51#, Fucheng Road, Haidian District, Beijing 100048, China; Department of Pharmacy, General Hospital of Beijing Military Region, 5 Nanmencang Road, Dong Cheng District, Beijing 100700, China. 3. Wounds Repair and Tissue Regeneration Laboratory, The First Affiliated Hospital, General Hospital of PLA, 51#, Fucheng Road, Haidian District, Beijing 100048, China. Electronic address: zcp666666@sohu.com. 4. Wounds Repair and Tissue Regeneration Laboratory, The First Affiliated Hospital, General Hospital of PLA, 51#, Fucheng Road, Haidian District, Beijing 100048, China. Electronic address: fuxiaobing@vip.sina.com.
Abstract
BACKGROUND: Chronic wounds have become an increasing medical and economic problem of aging societies because they are difficult to manage. Skin grafting is an important treatment method for chronic wounds, which are refractory to conservative therapy. The technique involving epidermal cell suspensions was invented to enable the possibility of treating larger wounds with only a small piece of donor skin. Both uncultured and cultured autologous epidermal cell suspensions can be prepared and survive permanently on the wound bed. METHODS: A systematic search was conducted of EMBASE, Cochrane Library, PubMed and web of science by using Boolean search terms, from the establishment of the database until May 31, 2014. The bibliographies of all retrieved articles in English were searched. The search terms were: (epithelial cell suspension OR keratinocyte suspension) and chronic and wound. RESULTS: From the included, 6 studies are descriptive interventions and discussed the use of autologous keratinocyte suspension to treat 61 patients' chronic wound. The various methods of preparation of epidermal cell suspension are described. The advantages and shortcomings of different carriers for epidermal cell suspensions are also summarised. Both uncultured and cultured autologous epidermal cell suspensions have been used to treat chronic wounds. CONCLUSION: Although the limitations of these studies include the small number of patient populations with chronic wounds and many important problems that remain to be solved, autologous epidermal cell suspension is a promising treatment for chronic wounds.
BACKGROUND: Chronic wounds have become an increasing medical and economic problem of aging societies because they are difficult to manage. Skin grafting is an important treatment method for chronic wounds, which are refractory to conservative therapy. The technique involving epidermal cell suspensions was invented to enable the possibility of treating larger wounds with only a small piece of donor skin. Both uncultured and cultured autologous epidermal cell suspensions can be prepared and survive permanently on the wound bed. METHODS: A systematic search was conducted of EMBASE, Cochrane Library, PubMed and web of science by using Boolean search terms, from the establishment of the database until May 31, 2014. The bibliographies of all retrieved articles in English were searched. The search terms were: (epithelial cell suspension OR keratinocyte suspension) and chronic and wound. RESULTS: From the included, 6 studies are descriptive interventions and discussed the use of autologous keratinocyte suspension to treat 61 patients' chronic wound. The various methods of preparation of epidermal cell suspension are described. The advantages and shortcomings of different carriers for epidermal cell suspensions are also summarised. Both uncultured and cultured autologous epidermal cell suspensions have been used to treat chronic wounds. CONCLUSION: Although the limitations of these studies include the small number of patient populations with chronic wounds and many important problems that remain to be solved, autologous epidermal cell suspension is a promising treatment for chronic wounds.
Authors: Paula Pleguezuelos-Beltrán; Patricia Gálvez-Martín; Daniel Nieto-García; Juan Antonio Marchal; Elena López-Ruiz Journal: Bioact Mater Date: 2022-03-08
Authors: Ahmed T El-Serafi; Ibrahim El-Serafi; Ingrid Steinvall; Folke Sjöberg; Moustafa Elmasry Journal: Int J Mol Sci Date: 2022-07-19 Impact factor: 6.208