Literature DB >> 2670604

Detection of IgA protease from Haemophilus influenzae by immunoblotting.

T Tsuji1, R M Alborno, M Ehara, T Honda, T Miwatani.   

Abstract

IgA protease produced by various strains of Haemophilus influenzae can digest serum IgA and yield its fragments which can react with anti-IgA serum. We assayed IgA protease activity by detecting the digests of IgA by SDS-PAGE and immunoblotting. The digests were separated with SDS-PAGE, transferred to nitrocellulose membranes and detected with anti- (alpha chain of human IgA, its Fab and its Fc) immunoglobulin conjugated peroxidases. Using this method, we can determine which type of IgA protease is produced by various of H. influenzae strains. All the 20 strains isolated from respiratory tracts produced IgA protease.

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Year:  1989        PMID： 2670604     DOI： 10.1007/bf00156830

Source DB:  PubMed          Journal:  Eur J Epidemiol        ISSN： 0393-2990            Impact factor:   8.082


  11 in total

1.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

Review 2.  Immunoblotting and dot immunobinding--current status and outlook.

Authors:  H Towbin; J Gordon
Journal:  J Immunol Methods       Date:  1984-09-04       Impact factor: 2.303

Review 3.  The IgA1 proteases of pathogenic bacteria.

Authors:  A G Plaut
Journal:  Annu Rev Microbiol       Date:  1983       Impact factor: 15.500

Review 4.  Secretory immunity and the bacterial IgA proteases.

Authors:  S J Kornfeld; A G Plaut
Journal:  Rev Infect Dis       Date:  1981 May-Jun

5.  Studies on gonococcus infection. XVII. IgA1-cleaving protease in vaginal washings from women with gonorrhea.

Authors:  M Blake; K K Holmes; J Swanson
Journal:  J Infect Dis       Date:  1979-01       Impact factor: 5.226

6.  Studies on gonococcus infection. XVI. Purification of Neisseria gonorrhoeae immunoglobulin A1 protease.

Authors:  M S Blake; J Swanson
Journal:  Infect Immun       Date:  1978-11       Impact factor: 3.441

7.  Specific proteolysis of human IgA by Streptococcus pneumoniae and Haemophilus influenzae.

Authors:  M H Mulks; S J Kornfeld; A G Plaut
Journal:  J Infect Dis       Date:  1980-04       Impact factor: 5.226

8.  Degradation of human immunoglobulins by proteases from Streptococcus pneumoniae obtained from various human sources.

Authors:  M B Wikström; G Dahlén; B Kaijser; H Nygren
Journal:  Infect Immun       Date:  1984-04       Impact factor: 3.441

9.  IgA1 proteases from Haemophilus influenzae, Streptococcus pneumoniae, Neisseria meningitidis, and Streptococcus sanguis: comparative immunochemical studies.

Authors:  M Kilian; J Mestecky; R Kulhavy; M Tomana; W T Butler
Journal:  J Immunol       Date:  1980-06       Impact factor: 5.422

10.  Immunoglobulin A1 protease production by Haemophilus influenzae and Streptococcus pneumoniae.

Authors:  C J Male
Journal:  Infect Immun       Date:  1979-10       Impact factor: 3.441
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  1 in total

1.  Bacillus anthracis Overcomes an Amino Acid Auxotrophy by Cleaving Host Serum Proteins.

Authors:  Austen Terwilliger; Michelle C Swick; Kathryn J Pflughoeft; Andrei Pomerantsev; C Rick Lyons; Theresa M Koehler; Anthony Maresso
Journal:  J Bacteriol       Date:  2015-05-11       Impact factor: 3.490
  1 in total

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