Literature DB >> 2670483

Histone H1 in the centromeric heterochromatin of Glyptotendipes barbipes larval polytene chromosomes.

S G Nonchev1, P V Michailova, C D Venkov, R G Tsanev.   

Abstract

Immunofluorescent analysis with antibodies against histone H1 failed to detect H1 in the centromeric heterochromatin blocks of the polytene chromosomes of Glyptotendipes barbipes larvae. Centromeric regions were dissected microsurgically and acid-extracted. Electrophoresis in SDS and acid-urea gels revealed a band comigrating with H1 of calf thymus and of Gl. barbipes salivary gland nuclei. ELISA dot assay of the extracted material gave a positive reaction with anti-H1 monoclonal antibodies and with anti-H1 affinity-purified polyclonal antibodies. This shows that the centromeric heterochromatin contains histone H1 but packed in a way which prevents the H1 antigenic determinants from reacting in situ with the specific antibodies.

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Year:  1989        PMID： 2670483     DOI： 10.1007/bf00293336

Source DB:  PubMed          Journal:  Chromosoma        ISSN： 0009-5915            Impact factor:   4.316


  21 in total

1.  Inversion polymorphism in the midge Glyptotendipes barbipes (Staeger).

Authors:  V R BASRUR
Journal:  Chromosoma       Date:  1957       Impact factor: 4.316

2.  Involvement of histone H1 in the organization of the chromosome fiber.

Authors:  M Renz; P Nehls; J Hozier
Journal:  Proc Natl Acad Sci U S A       Date:  1977-05       Impact factor: 11.205

3.  Immunofluorescent localization of histone H10 in the nuclei of proliferating and differentiating Friend cells.

Authors:  T Banchev; L Srebreva; J Zlatanova; R Tsanev
Journal:  Exp Cell Res       Date:  1988-07       Impact factor: 3.905

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  A method for determination of the in situ distribution of chromosomal proteins.

Authors:  L M Silver; S C Elgin
Journal:  Proc Natl Acad Sci U S A       Date:  1976-02       Impact factor: 11.205

6.  Localization of nuclear proteins related to high mobility group protein 14 (HMG 14) in polytene chromosomes.

Authors:  R Westermann; U Grossbach
Journal:  Chromosoma       Date:  1984       Impact factor: 4.316

7.  Histone H1 heterogeneity in the midge, Chironomus thummi. Structural comparison of the H1 variants in an organism where their intrachromosomal localization is possible.

Authors:  S Hoyer-Fender; U Grossbach
Journal:  Eur J Biochem       Date:  1988-09-01

8.  Two AT-rich satellite DNAs in the chironomid Glyptotendipes barbipes (Staeger): isolation and localization in polytene chromosomes of G. barbipes and Chironomus thummi.

Authors:  E R Schmidt
Journal:  Chromosoma       Date:  1980       Impact factor: 4.316

9.  Localization of chromosomal protein HMG-1 in polytene chromosomes of Chironomus thummi.

Authors:  P D Kurth; M Bustin
Journal:  J Cell Biol       Date:  1981-04       Impact factor: 10.539

10.  Histone localization in polytene chromosomes by immunofluorescence.

Authors:  P D Kurth; E N Moudrianakis; M Bustin
Journal:  J Cell Biol       Date:  1978-09       Impact factor: 10.539
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  3 in total

Review 1.  The evolution of small DNA viruses of eukaryotes: past and present considerations.

Authors:  F F Shadan; L P Villarreal
Journal:  Virus Genes       Date:  1995       Impact factor: 2.332

2.  Bands, interbands and puffs in native Drosophila polytene chromosomes are recognized by a monoclonal antibody to an epitope in the carboxy-terminal tail of histone H1.

Authors:  R J Hill; F Watt; C M Wilson; T Fifis; P A Underwood; G Tribbick; H M Geysen; J O Thomas
Journal:  Chromosoma       Date:  1989-12       Impact factor: 4.316

3.  Structurally divergent histone H1 variants in chromosomes containing highly condensed interphase chromatin.

Authors:  E Schulze; S Nagel; K Gavenis; U Grossbach
Journal:  J Cell Biol       Date:  1994-12       Impact factor: 10.539
  3 in total

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