Satoru Horigome1,2, Izumi Yoshida2, Shihomi Ito2, Shuichi Inohana2, Kei Fushimi2, Takeshi Nagai2, Akihiro Yamaguchi2,3, Kazuhiro Fujita2, Toshiya Satoyama2,4, Shin-Ichi Katsuda2, Shinobu Suzuki2, Masatoshi Watai2, Naoto Hirose5, Takahiro Mitsue6, Hitoshi Shirakawa7, Michio Komai1. 1. Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan. 2. Japan Food Research Laboratories, 6-11-10, Nagayama, Tama, Tokyo, 206-0025, Japan. 3. Department of Food Science and Human Wellness, Rakuno Gakuen University, 582, Bunkyodai Midorimachi, Ebetsu, Hokkaido, 069-8501, Japan. 4. Research and Development Office, Kubara Honke Shokuhin Co., Ltd., 1442, Ino, Hisayama-machi, Kasuya-gun, Fukuoka, 811-2503, Japan. 5. Okinawa Prefectural Agricultural Research Center, 820, Makabe, Itoman, Okinawa, 901-0336, Japan. 6. Department of Bioresources Engineering, Okinawa National College of Technology, 905 Henoko, Nago, Okinawa, 905-2192, Japan. 7. Laboratory of Nutrition, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-Amamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan. shirakah@m.tohoku.ac.jp.
Abstract
PURPOSE: The rhizome of Kaempferia parviflora (KP) is used in traditional Thai medicine. In this study, we investigated the effects of an ethanol KP extract and two of its components [5,7-dimethoxyflavone (DMF) and 5-hydroxy-3,7,3',4'-tetramethoxyflavone (TMF)] on monocyte adhesion and cellular reactive oxygen species (ROS) production in human umbilical vein endothelial cells (HUVECs), which provide an in vitro model of events relevant to the development and progression of atherosclerosis. METHODS: RAW264.7 mouse macrophage-like cells were incubated with various concentrations of KP extract or polymethoxyflavonoids and stimulated with lipopolysaccharide prior to measuring nitrite levels in the culture media. Monocyte adhesion was evaluated by measuring the fluorescently labeled human monocytic leukemia THP-1 cells that is attached to tumor necrosis factor-α (TNF-α)-stimulated HUVECs. Cellular ROS production was assessed by measuring cellular antioxidant activity using pyocyanin-stimulated HUVECs. RESULTS: KP extract and DMF reduced nitrite levels (as indicator of nitric oxide production) in LPS-stimulated RAW264.7 cells and also inhibited THP-1 cell adhesion to HUVECs. These treatments induced mRNA expression of endothelial nitric oxide synthase in TNF-α-stimulated HUVECs and downregulated that of various cell adhesion molecules, inflammatory mediators, and endothelial function-related genes. Angiotensin-converting enzyme activity was inhibited by KP extract in vitro. Furthermore, KP extract, DMF, and TMF inhibited the production of cellular ROS in pyocyanin-stimulated HUVECs. CONCLUSION: KP extract, DMF, and TMF showed potential anti-inflammatory and antioxidant effects in these in vitro models, properties that would inhibit the development and progression of atherosclerosis.
PURPOSE: The rhizome of Kaempferia parviflora (KP) is used in traditional Thai medicine. In this study, we investigated the effects of an ethanolKP extract and two of its components [5,7-dimethoxyflavone (DMF) and 5-hydroxy-3,7,3',4'-tetramethoxyflavone (TMF)] on monocyte adhesion and cellular reactive oxygen species (ROS) production in human umbilical vein endothelial cells (HUVECs), which provide an in vitro model of events relevant to the development and progression of atherosclerosis. METHODS: RAW264.7 mouse macrophage-like cells were incubated with various concentrations of KP extract or polymethoxyflavonoids and stimulated with lipopolysaccharide prior to measuring nitrite levels in the culture media. Monocyte adhesion was evaluated by measuring the fluorescently labeled human monocytic leukemia THP-1 cells that is attached to tumor necrosis factor-α (TNF-α)-stimulated HUVECs. Cellular ROS production was assessed by measuring cellular antioxidant activity using pyocyanin-stimulated HUVECs. RESULTS:KP extract and DMF reduced nitrite levels (as indicator of nitric oxide production) in LPS-stimulated RAW264.7 cells and also inhibited THP-1 cell adhesion to HUVECs. These treatments induced mRNA expression of endothelial nitric oxide synthase in TNF-α-stimulated HUVECs and downregulated that of various cell adhesion molecules, inflammatory mediators, and endothelial function-related genes. Angiotensin-converting enzyme activity was inhibited by KP extract in vitro. Furthermore, KP extract, DMF, and TMF inhibited the production of cellular ROS in pyocyanin-stimulated HUVECs. CONCLUSION:KP extract, DMF, and TMF showed potential anti-inflammatory and antioxidant effects in these in vitro models, properties that would inhibit the development and progression of atherosclerosis.
Authors: Oliver Soehnlein; Alexander Schmeisser; Iwona Cicha; Christine Reiss; Holger Ulbrich; Lennart Lindbom; Werner G Daniel; Christoph D Garlichs Journal: J Vasc Res Date: 2005-08-05 Impact factor: 1.934
Authors: Katarzyna Oszajca; Magdalena Bieniasz; George Brown; Maria Swiatkowska; Jacek Bartkowiak; Janusz Szemraj Journal: Biochem Cell Biol Date: 2008-12 Impact factor: 3.626
Authors: Christian Schwarzer; Horst Fischer; Eun-Jin Kim; Katharine J Barber; Aaron D Mills; Mark J Kurth; Dieter C Gruenert; Jung H Suh; Terry E Machen; Beate Illek Journal: Free Radic Biol Med Date: 2008-09-23 Impact factor: 7.376