Erica E Marsh1, Shani Chibber2, Ju Wu3, Kendra Siegersma3, Julie Kim3, Serdar Bulun4. 1. Division of Reproductive Science and Medicine, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois. Electronic address: erica-marsh@northwestern.edu. 2. Division of Reproductive Science and Medicine, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois; University of Illinois College of Medicine, Chicago, Illinois. 3. Division of Reproductive Science and Medicine, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois. 4. Division of Reproductive Science and Medicine, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois; Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, Feinberg School of Medicine, Northwestern University, Chicago, Illinois.
Abstract
OBJECTIVE: To determine the presence, differential expression, and regulation of epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) in uterine leiomyomas. DESIGN: Laboratory in vivo and in vitro study with the use of human leiomyoma and myometrial tissue and primary cells. SETTING: Academic medical center. SAMPLE(S): Leiomyoma and myometrial tissue samples and cultured cells. INTERVENTION(S): 5-Aza-2'-deoxycytidine (5-aza-dC) treatment. MAIN OUTCOME MEASURE(S): Fold-change difference between EFEMP1 and fibulin-3 expression in leiomyoma tissue and cells compared with matched myometrial samples, and fold-change difference in EFEMP1 expression with 5-Aza-dC treatment. RESULT(S): In vivo, EFEMP1 expression was 3.19-fold higher in myometrial tissue than in leiomyoma tissue. EFEMP1 expression in vitro was 5.03-fold higher in myometrial cells than in leiomyoma cells. Western blot and immunohistochemistry staining of tissue and cells confirmed similar findings in protein expression. Treatment of leiomyoma cells with 5-Aza-dC resulted in increased expression of EFEMP1 in vitro. CONCLUSION(S): The EFEMP1 gene and its protein product, fibulin-3, are both significantly down-regulated in leiomyoma compared with myometrium when studied both in vivo and in vitro. The increase in EFEMP1 expression in leiomyoma cells with 5-Aza-dC treatment suggest that differential methylation is responsible, in part, for the differences seen in gene expression.
OBJECTIVE: To determine the presence, differential expression, and regulation of epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) in uterine leiomyomas. DESIGN: Laboratory in vivo and in vitro study with the use of humanleiomyoma and myometrial tissue and primary cells. SETTING: Academic medical center. SAMPLE(S): Leiomyoma and myometrial tissue samples and cultured cells. INTERVENTION(S): 5-Aza-2'-deoxycytidine (5-aza-dC) treatment. MAIN OUTCOME MEASURE(S): Fold-change difference between EFEMP1 and fibulin-3 expression in leiomyoma tissue and cells compared with matched myometrial samples, and fold-change difference in EFEMP1 expression with 5-Aza-dC treatment. RESULT(S): In vivo, EFEMP1 expression was 3.19-fold higher in myometrial tissue than in leiomyoma tissue. EFEMP1 expression in vitro was 5.03-fold higher in myometrial cells than in leiomyoma cells. Western blot and immunohistochemistry staining of tissue and cells confirmed similar findings in protein expression. Treatment of leiomyoma cells with 5-Aza-dC resulted in increased expression of EFEMP1 in vitro. CONCLUSION(S): The EFEMP1 gene and its protein product, fibulin-3, are both significantly down-regulated in leiomyoma compared with myometrium when studied both in vivo and in vitro. The increase in EFEMP1 expression in leiomyoma cells with 5-Aza-dC treatment suggest that differential methylation is responsible, in part, for the differences seen in gene expression.
Authors: David W Lee; Teresa B Gibson; Ginger S Carls; Ronald J Ozminkowski; Shaohung Wang; Elizabeth A Stewart Journal: Fertil Steril Date: 2008-03-04 Impact factor: 7.329
Authors: Samantha B Schon; Nicholas Raja; Min Xu; Heather Cameron; Kun Yang; Jayne Reynolds; Dee Fenner; Erica E Marsh Journal: J Assist Reprod Genet Date: 2021-03-30 Impact factor: 3.357