Gustavo Costa1, João Pinto Ferreira2, Carla Vitorino2, Maria Eugénia Pina3, João José Sousa2, Isabel Vitória Figueiredo4, Maria Teresa Batista2. 1. Center for Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Portugal; Center for Neurosciences and Cell Biology, University of Coimbra, Portugal. Electronic address: costagff@gmail.com. 2. Center for Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Portugal; Center for Neurosciences and Cell Biology, University of Coimbra, Portugal. 3. CIEPQPF, Research Center for Chemical Processes Engineering and Forest Products, Chemical Engineering Department, Faculty of Sciences and Technology, University of Coimbra, Portugal. 4. Center for Pharmaceutical Studies, Faculty of Pharmacy, University of Coimbra, Portugal; Institute for Biomedical Imaging and Life Sciences, University of Coimbra, Portugal.
Abstract
ETHNOPHARMACOLOGICAL RELEVANCE: A variety of plant polyphenols have been reported to have anti-inflammatory, frequently associated with erythema, edema, hyperplasia, skin photoaging and photocarcinogenesis. Cymbopogon citratus (DC). Stapf (Poaceae) is a worldwide known medicinal plant, used in traditional medicine in inflammation-related conditions. AIM OF THE STUDY: In this work, the anti-inflammatory potential of C. citratus infusion (CcI) and its polyphenols as topical agents was evaluated in vivo. MATERIALS AND METHODS: The plant extract was prepared and its fractioning led two polyphenol-rich fractions: flavonoids fraction (CcF) and tannins fraction (CcT). An oil/water emulsion was developed with each active (CcI, CcF+CcT and diclofenac), pH and texture having been evaluated. Release tests were further performed using static Franz diffusion cells and all collected samples were monitored by HPLC-PDA. In vivo topical anti-inflammatory activity evaluation was performed by the carrageenan-induced rat paw edema model. RESULTS: The texture analysis revealed statistically significant differences for all tested parameters to CcF+CcT, supporting its topical application. Release experiments lead to the detection of the phenolic compounds from each sample in the receptor medium and the six major flavonoids were quantified, by HPLC-PDA: carlinoside, isoorientin, cynaroside, luteolin 7-O-neohesperidoside, kurilesin A and cassiaoccidentalin B. The CcF+CcT formulation prompted to the higher release rate for all these flavonoids. CcI4%, CcI1% and CcF+CcT exhibited an edema reduction of 43.18, 29.55 and 59.09%, respectively. CONCLUSIONS: Our findings highlight that CcI, containing luteolin 7-O-neohesperidoside, cassiaoccidentalin B, carlinoside, cynaroside and tannins have a potential anti-inflammatory topical activity, suggesting their promising application in the treatment of skin inflammatory pathologies.
ETHNOPHARMACOLOGICAL RELEVANCE: A variety of plant polyphenols have been reported to have anti-inflammatory, frequently associated with erythema, edema, hyperplasia, skin photoaging and photocarcinogenesis. Cymbopogon citratus (DC). Stapf (Poaceae) is a worldwide known medicinal plant, used in traditional medicine in inflammation-related conditions. AIM OF THE STUDY: In this work, the anti-inflammatory potential of C. citratus infusion (CcI) and its polyphenols as topical agents was evaluated in vivo. MATERIALS AND METHODS: The plant extract was prepared and its fractioning led two polyphenol-rich fractions: flavonoids fraction (CcF) and tannins fraction (CcT). An oil/water emulsion was developed with each active (CcI, CcF+CcT and diclofenac), pH and texture having been evaluated. Release tests were further performed using static Franz diffusion cells and all collected samples were monitored by HPLC-PDA. In vivo topical anti-inflammatory activity evaluation was performed by the carrageenan-induced rat paw edema model. RESULTS: The texture analysis revealed statistically significant differences for all tested parameters to CcF+CcT, supporting its topical application. Release experiments lead to the detection of the phenolic compounds from each sample in the receptor medium and the six major flavonoids were quantified, by HPLC-PDA: carlinoside, isoorientin, cynaroside, luteolin 7-O-neohesperidoside, kurilesin A and cassiaoccidentalin B. The CcF+CcT formulation prompted to the higher release rate for all these flavonoids. CcI4%, CcI1% and CcF+CcT exhibited an edema reduction of 43.18, 29.55 and 59.09%, respectively. CONCLUSIONS: Our findings highlight that CcI, containing luteolin 7-O-neohesperidoside, cassiaoccidentalin B, carlinoside, cynaroside and tannins have a potential anti-inflammatory topical activity, suggesting their promising application in the treatment of skin inflammatory pathologies.
Authors: Sónia Pedreiro; Sandrine da Ressurreição; Maria Lopes; Maria Teresa Cruz; Teresa Batista; Artur Figueirinha; Fernando Ramos Journal: Int J Environ Res Public Health Date: 2020-12-28 Impact factor: 3.390
Authors: Hosam O Elansary; Agnieszka Szopa; Paweł Kubica; Halina Ekiert; Hayssam M Ali; Mohamed S Elshikh; Eslam M Abdel-Salam; Mohamed El-Esawi; Diaa O El-Ansary Journal: Evid Based Complement Alternat Med Date: 2018-01-31 Impact factor: 2.629