OBJECTIVE: To observe mitophagy in APPsw/PS1dE9 transgenic mice and APPsw stably expressing HEK293 cell. MATERIALS AND METHODS: The APPsw/PS1dE9 transgenic mice and 20E2 cell (HEK293 cell with APPsw) was used to be the model of Alzheimer's disease. We dynamically observed the behavior, mitochondrial structure and mitophagy in brain of APP/PS1 transgenic mice in different age groups. Mitochondrial structure and mitophagy of HEK293 and 20E2 cell in vitro were also recorded. RESULTS: The mitochondrion was changed significantly in APP/PS1 transgenic mice and 20E2 cell. LC3 protein, associated with autophagy, was up-regulated. Mitophagy related protein PINK1 and PARKIN were up-regulated. CONCLUSIONS: Mitophagy disorder may be associated with the pathogenesis of Alzheimer's Disease through PINK1/Parkin pathway.
OBJECTIVE: To observe mitophagy in APPsw/PS1dE9 transgenic mice and APPsw stably expressing HEK293 cell. MATERIALS AND METHODS: The APPsw/PS1dE9 transgenic mice and 20E2 cell (HEK293 cell with APPsw) was used to be the model of Alzheimer's disease. We dynamically observed the behavior, mitochondrial structure and mitophagy in brain of APP/PS1transgenic mice in different age groups. Mitochondrial structure and mitophagy of HEK293 and 20E2 cell in vitro were also recorded. RESULTS: The mitochondrion was changed significantly in APP/PS1transgenic mice and 20E2 cell. LC3 protein, associated with autophagy, was up-regulated. Mitophagy related protein PINK1 and PARKIN were up-regulated. CONCLUSIONS:Mitophagy disorder may be associated with the pathogenesis of Alzheimer's Disease through PINK1/Parkin pathway.
Authors: Stephani A Davis; Sheed Itaman; Christopher M Khalid-Janney; Justin A Sherard; James A Dowell; Nigel J Cairns; Michael A Gitcho Journal: Neurosci Lett Date: 2018-04-30 Impact factor: 3.046