Literature DB >> 26695017

Purification of Na,K-ATPase from Pig Kidney.

Natalya U Fedosova1,2.   

Abstract

The method of purification of Na,K-ATPase from pig kidney is based on a differential centrifugation and SDS-treatment of a microsomal preparation. The yield is 0.4 mg protein per 1 g tissue with the specific (ouabain-sensitive) activity of 25-28 μmol Pi/min per mg protein and nucleotide binding capacity of 3 nmol/mg. The protein/lipid ratio is 1/1 (mg/mg) with a protein purity of ~80 %.

Entities:  

Keywords:  Kidney; Microsomal fraction differential centrifugation; Na,K-ATPase; SDS -treatment

Mesh：

Substances：

Year:  2016        PMID： 26695017     DOI： 10.1007/978-1-4939-3179-8_2

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  2 in total

1.  Anti-Na+/K+-ATPase immunotherapy ameliorates α-synuclein pathology through activation of Na+/K+-ATPase α1-dependent autophagy.

Authors:  Lei Cao; Siping Xiong; Zhiyuan Wu; Lei Ding; Yebo Zhou; Haijian Sun; Mengyuan Zhu; Wei Thye Lee; Xiaowei Nie; Jin-Song Bian
Journal:  Sci Adv       Date:  2021-01-27       Impact factor: 14.136

2.  Procyanidin C1 from Viola odorata L. inhibits Na+,K+-ATPase.

Authors:  Tomas Heger; Marek Zatloukal; Martin Kubala; Miroslav Strnad; Jiri Gruz
Journal:  Sci Rep       Date:  2022-04-29       Impact factor: 4.996
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.