BACKGROUND: Aflatoxin B1 (AFB1 ) is often detected in peanut oil, which comes from contaminated peanuts. AFB1 in peanut oil seriously threatens the health of consumers. However, there are few methods to effectively remove AFB1 in peanut oil. This study aimed to use an alkali-refining method to degrade AFB1 in peanut oil efficiently without increasing the equipment of oil and fat refining. RESULTS: The optimum detoxifying conditions of AFB1 in peanut oil with alkali refining were established using response surface methodology (RSM), and the safety of peanut oil after being refined with alkali was evaluated based on the Ames tests and HepG2 cell viability. The results showed that AFB1 in peanut oil was decreased from 34.78 to 0.37 µg kg(-1) (98.94% reduction) under the optimum detoxifying conditions, i.e. when the initial temperature of alkali refining was 43.51 °C, the amount of excess alkali was 0.30%, the content of alkali solution was 23.42% and the end temperature of alkali refining was 77.07 °C. The acid value and color of peanut oil refined by alkali were improved significantly, while the peroxide value was increased within an acceptable level. The safety of peanut oil contaminated by AFB1 was improved significantly after being refined with alkali. CONCLUSION: These results indicate that alkali refining is an effective method for removing AFB1 in peanut oil. The optimum detoxifying conditions of AFB1 in peanut oil with alkali refining could be used to guide the production of oil companies for ensuring food safety.
BACKGROUND:Aflatoxin B1 (AFB1 ) is often detected in peanutoil, which comes from contaminated peanuts. AFB1 in peanutoil seriously threatens the health of consumers. However, there are few methods to effectively remove AFB1 in peanutoil. This study aimed to use an alkali-refining method to degrade AFB1 in peanutoil efficiently without increasing the equipment of oil and fat refining. RESULTS: The optimum detoxifying conditions of AFB1 in peanutoil with alkali refining were established using response surface methodology (RSM), and the safety of peanutoil after being refined with alkali was evaluated based on the Ames tests and HepG2 cell viability. The results showed that AFB1 in peanutoil was decreased from 34.78 to 0.37 µg kg(-1) (98.94% reduction) under the optimum detoxifying conditions, i.e. when the initial temperature of alkali refining was 43.51 °C, the amount of excess alkali was 0.30%, the content of alkali solution was 23.42% and the end temperature of alkali refining was 77.07 °C. The acid value and color of peanutoil refined by alkali were improved significantly, while the peroxide value was increased within an acceptable level. The safety of peanutoil contaminated by AFB1 was improved significantly after being refined with alkali. CONCLUSION: These results indicate that alkali refining is an effective method for removing AFB1 in peanutoil. The optimum detoxifying conditions of AFB1 in peanutoil with alkali refining could be used to guide the production of oil companies for ensuring food safety.