Detection of Cellulose-Derived Chromophores by Ambient Ionization-MS.

The detection of individual chromophores that contribute to the overall discoloration of paper ("yellowing") is a challenge because those substances are only present in very small amounts. In this research, two analytical approaches based on ambient ionization techniques, namely, desorption electrospray ionization and paper spray, both coupled to mass spectrometry, are compared to each other with regard to their suitability for detecting acetylated cellulosic key chromophores. The paper spray approach proved to be the more sensitive and versatile method. Subsequently, paper spray (PS)-mass spectrometry was applied to model papers and historical papers in which the acetylated chromophores were detected successfully. Independent accurate mass measurements confirmed the results obtained from reference compounds, model samples, and real-world specimens.