Doh Young Lee1, Su A Park2, Sang Jin Lee3,2, Tae Ho Kim4, Se Heang Oh5, Jin Ho Lee4, Seong Keun Kwon6. 1. Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Republic of Korea. 2. Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials, Daejeon, Republic of Korea. 3. Department of Maxillofacial Biomedical Engineering and Institute of Oral Biology, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea. 4. Department of Advanced Materials, Hannam University, Daejeon, Republic of Korea. 5. Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, Republic of Korea. 6. Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University Hospital, Seoul, Republic of Korea.
Abstract
OBJECTIVES/HYPOTHESIS: Three-dimensional (3D) printed scaffold for tracheal reconstruction can substitute the conventional treatment of tracheal stenosis. This study investigated the survival outcomes of segmental tracheal reconstruction using 3D printed polycaprolactone (PCL) scaffold with or without asymmetrically porous membrane in rabbit animal model. STUDY DESIGN: Animal study. METHODS: Six mature New Zealand white rabbits were categorized into two groups (three animals for each) according to the procedures they received: tracheal reconstruction using 3D printed PCL scaffold without asymmetrically porous membrane (group 1) versus with asymmetrically porous membrane (group 2). We compared the endoscopic findings of tracheal lumen, radiologic assessment using microcomputed tomography (CT) scanner and histologic findings. Overall survival duration after procedure was compared in both groups. RESULTS: The survival of group 2 was longer than group 1 (21, 37, 46 days vs. 4, 10, 12 days, respectively). Although mucosal regeneration in tracheal lumen was not full enough in both groups, the patency was well maintained in group 2. Micro-CT and histologic analysis showed that there were tracheal narrowing in the whole length in group 1, whereas only the anastomosis site was stenotic in group 2. CONCLUSION: Asymmetrically porous membrane reinforced by 3D printed mesh is promising as a 360-degree tracheal substitute with comparable survival and luminal patency. Further study is necessary to minimize the narrowing of the anastomosis site and improve the mucosal regeneration for longer survival. LEVEL OF EVIDENCE: NA. Laryngoscope, 126:E304-E309, 2016.
OBJECTIVES/HYPOTHESIS: Three-dimensional (3D) printed scaffold for tracheal reconstruction can substitute the conventional treatment of tracheal stenosis. This study investigated the survival outcomes of segmental tracheal reconstruction using 3D printed polycaprolactone (PCL) scaffold with or without asymmetrically porous membrane in rabbit animal model. STUDY DESIGN: Animal study. METHODS: Six mature New Zealand white rabbits were categorized into two groups (three animals for each) according to the procedures they received: tracheal reconstruction using 3D printed PCL scaffold without asymmetrically porous membrane (group 1) versus with asymmetrically porous membrane (group 2). We compared the endoscopic findings of tracheal lumen, radiologic assessment using microcomputed tomography (CT) scanner and histologic findings. Overall survival duration after procedure was compared in both groups. RESULTS: The survival of group 2 was longer than group 1 (21, 37, 46 days vs. 4, 10, 12 days, respectively). Although mucosal regeneration in tracheal lumen was not full enough in both groups, the patency was well maintained in group 2. Micro-CT and histologic analysis showed that there were tracheal narrowing in the whole length in group 1, whereas only the anastomosis site was stenotic in group 2. CONCLUSION: Asymmetrically porous membrane reinforced by 3D printed mesh is promising as a 360-degree tracheal substitute with comparable survival and luminal patency. Further study is necessary to minimize the narrowing of the anastomosis site and improve the mucosal regeneration for longer survival. LEVEL OF EVIDENCE: NA. Laryngoscope, 126:E304-E309, 2016.
Authors: Hae Sang Park; Hyun Jung Park; Junhee Lee; Pureum Kim; Ji Seung Lee; Young Jin Lee; Ye Been Seo; Do Yeon Kim; Olatunji Ajiteru; Ok Joo Lee; Chan Hum Park Journal: Tissue Eng Regen Med Date: 2018-07-14 Impact factor: 4.169