Literature DB >> 2668749

A sensitive method for the detection of mutagenic nitroarenes: construction of nitroreductase-overproducing derivatives of Salmonella typhimurium strains TA98 and TA100.

M Watanabe1, M Ishidate, T Nohmi.   

Abstract

'Classical nitroreductase' is an enzyme involved in the intracellular metabolic activation of mutagenic nitroarenes. The nitroreductase gene of Salmonella typhimurium TA1538 was cloned into pBR322 and the plasmids harboring the gene were introduced into TA98 and TA100. The resulting strains (YG1021 and YG1026) had more than 50 times higher nitrofurazone-reductase activity than TA1538 containing pBR322, and were extremely sensitive to the mutagenic action of 2-nitrofluorene, 1-nitropyrene and 2-nitronaphthalene. These results indicate that the new strains permit the efficient detection of mutagenic nitroarenes.

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Year:  1989        PMID： 2668749     DOI： 10.1016/0165-1161(89)90007-1

Source DB:  PubMed          Journal:  Mutat Res        ISSN： 0027-5107            Impact factor:   2.433


  10 in total

1.  Nucleotide sequence of Salmonella typhimurium nitroreductase gene.

Authors:  M Watanabe; M Ishidate; T Nohmi
Journal:  Nucleic Acids Res       Date:  1990-02-25       Impact factor: 16.971

2.  Identification of a Mycobacterium tuberculosis putative classical nitroreductase gene whose expression is coregulated with that of the acr aene within macrophages, in standing versus shaking cultures, and under low oxygen conditions.

Authors:  Anjan Purkayastha; Lee Ann McCue; Kathleen A McDonough
Journal:  Infect Immun       Date:  2002-03       Impact factor: 3.441

3.  Identification of the gene encoding the major NAD(P)H-flavin oxidoreductase of the bioluminescent bacterium Vibrio fischeri ATCC 7744.

Authors:  S Zenno; K Saigo; H Kanoh; S Inouye
Journal:  J Bacteriol       Date:  1994-06       Impact factor: 3.490

4.  New method for gene disruption in Salmonella typhimurium: construction and characterization of an ada-deletion derivative of Salmonella typhimurium TA1535.

Authors:  M Yamada; A Hakura; T Sofuni; T Nohmi
Journal:  J Bacteriol       Date:  1993-09       Impact factor: 3.490

5.  Involvement of umuDCST genes in nitropyrene-induced -CG frameshift mutagenesis at the repetitive CG sequence in the hisD3052 allele of Salmonella typhimurium.

Authors:  T Nohmi; M Yamada; M Matsui; K Matsui; M Watanabe; T Sofuni
Journal:  Mol Gen Genet       Date:  1995-04-10

6.  Purification and characterization of nitrobenzene nitroreductase from Pseudomonas pseudoalcaligenes JS45.

Authors:  C C Somerville; S F Nishino; J C Spain
Journal:  J Bacteriol       Date:  1995-07       Impact factor: 3.490

7.  Biomarkers for exposure to ambient air pollution--comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress.

Authors:  H Autrup; B Daneshvar; L O Dragsted; M Gamborg; M Hansen; S Loft; H Okkels; F Nielsen; P S Nielsen; E Raffn; H Wallin; L E Knudsen
Journal:  Environ Health Perspect       Date:  1999-03       Impact factor: 9.031

8.  Bioassay-directed fractionation and salmonella mutagenicity of automobile and forklift diesel exhaust particles.

Authors:  David M DeMarini; Lance R Brooks; Sarah H Warren; Takahiro Kobayashi; M Ian Gilmour; Pramila Singh
Journal:  Environ Health Perspect       Date:  2004-06       Impact factor: 9.031

9.  Mutagenicity of an aged gasworks soil during bioslurry treatment.

Authors:  Christine L Lemieux; Krista D Lynes; Paul A White; Staffan Lundstedt; Lars Oberg; Iain B Lambert
Journal:  Environ Mol Mutagen       Date:  2009-06       Impact factor: 3.216

10.  Genotoxicity of polycyclic aromatic hydrocarbons and nitro-derived in respirable airborne particulate matter collected from urban areas of Rio de Janeiro (Brazil).

Authors:  Claudia Ramos de Rainho; Sérgio Machado Corrêa; José Luiz Mazzei; Claudia Alessandra Fortes Aiub; Israel Felzenszwalb
Journal:  Biomed Res Int       Date:  2013-05-02       Impact factor: 3.411
  10 in total

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