Luciana Strieder1, Román Carlos2, Jorge Esquiche León3, Alfredo Ribeiro-Silva4, Victor Costa1, Estela Kaminagakura5. 1. Master Student of Oral Biopathology, Institute of Science and Technology, UNESP-Univ Estadual Paulista, School of Dentistry, São José dos Campos, Brazil. 2. Director of Centro Clínico de Cabeza y Cuello, Guatemala City, Guatemala. 3. Professor of Oral Pathology, Department of Stomatology, Public Oral Health and Forensic Dentistry, University of São Paulo, Ribeirão Preto School of Dentistry, Ribeirão Preto, Brazil. 4. Department of Pathology, Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil. 5. Professor of Stomatology, Department of Bioscience and Oral Diagnosis, Institute of Science and Technology, UNESP-Univ Estadual Paulista, School of Dentistry, São José dos Campos, Brazil. Electronic address: estela@fosjc.unesp.br.
Abstract
OBJECTIVE: The aim of this study is to report 2 cases of melanotic neuroectodermal tumor of infancy (MNTI), emphasizing the analysis of intratumoral immune cells by immunohistochemistry. STUDY DESIGN: Case 1: A 6-month-old girl presented with a 3-cm tumor in the anterior region of the left maxilla. Case 2: A 4-month-old boy presented with a 4-cm tumor in the anterior region of the left maxilla. Microscopically, case 1 had predominantly neuroblast-like cells supported by fibrillary neuropil-like stroma arranged in an alveolar pattern, whereas case 2 exhibited scattered melanocyte-like and neuroblast-like cells supported by fibrovascular stroma. A large immunohistochemical panel for characterizing intratumoral macrophage and dendritic cell subsets was performed. RESULTS: Immunohistochemical analysis indicated positivity for HLA-DR, XIIIa, CD68, and CD163 (range 6%-50%) mainly on the fibrovascular stroma, suggesting M2 macrophage-like cell phenotype. CD138 was overexpressed in the tumor stroma. CONCLUSIONS: Results suggest the involvement of M2-polarized macrophages in the MNTI pathogenesis, which may act by modulating tumor growth and/or tumor stromal remodeling.
OBJECTIVE: The aim of this study is to report 2 cases of melanotic neuroectodermal tumor of infancy (MNTI), emphasizing the analysis of intratumoral immune cells by immunohistochemistry. STUDY DESIGN: Case 1: A 6-month-old girl presented with a 3-cm tumor in the anterior region of the left maxilla. Case 2: A 4-month-old boy presented with a 4-cm tumor in the anterior region of the left maxilla. Microscopically, case 1 had predominantly neuroblast-like cells supported by fibrillary neuropil-like stroma arranged in an alveolar pattern, whereas case 2 exhibited scattered melanocyte-like and neuroblast-like cells supported by fibrovascular stroma. A large immunohistochemical panel for characterizing intratumoral macrophage and dendritic cell subsets was performed. RESULTS: Immunohistochemical analysis indicated positivity for HLA-DR, XIIIa, CD68, and CD163 (range 6%-50%) mainly on the fibrovascular stroma, suggesting M2 macrophage-like cell phenotype. CD138 was overexpressed in the tumor stroma. CONCLUSIONS: Results suggest the involvement of M2-polarized macrophages in the MNTI pathogenesis, which may act by modulating tumor growth and/or tumor stromal remodeling.
Authors: André Luca Araujo de Sousa; Wanderson Carvalho de Almeida; Jean de Pinho Mendes; Victor Angelo Martins Montalli; Antonione Santos Bezerra Pinto Journal: Acta Stomatol Croat Date: 2022-06