Literature DB >> 2668342

Comparison of Y1 mouse adrenal cell and coagglutination assays for detection of Escherichia coli heat labile enterotoxin.

P A Chapman1, C M Daly.   

Abstract

A commercial coagglutination assay (COA; Phadebact LT-ETEC) was compared with a Y1 mouse adrenal cell assay for detecting the heat labile enterotoxin of Escherichia coli. Of four different media evaluated for use with the COA, only one (modified blood agar) gave a positive result with all strains known to produce heat labile enterotoxin. With modified blood agar, the COA detected 74 (85%) of 87 such strains. Eighty six strains negative by cell culture assay were also negative by COA, and one strain positive by COA could not be confirmed by cell culture. The Phadebact LT-ETEC kit provides a simple, sensitive, and economical method for detecting E coli heat labile enterotoxin.

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Year:  1989        PMID： 2668342      PMCID： PMC1142029          DOI： 10.1136/jcp.42.7.755

Source DB:  PubMed          Journal:  J Clin Pathol        ISSN： 0021-9746            Impact factor:   3.411


  14 in total

1.  Enterotoxigenic Escherichia coli in Sweden.

Authors:  E Bück; S Blomberg; T Wadström
Journal:  Infection       Date:  1977       Impact factor: 3.553

2.  Frequency of ischaemic exercise E.C.G. Changes in symptom-free men with various forms of primary hyperlipaemia.

Authors:  L A Carlson; L G Ekelund; A G Olsson
Journal:  Lancet       Date:  1975-07-05       Impact factor: 79.321

3.  Nutritional requirements for synthesis of heat-labile enterotoxin by enterotoxigenic strains of Escherichia coli.

Authors:  P H Gilligan; D C Robertson
Journal:  Infect Immun       Date:  1979-01       Impact factor: 3.441

4.  Induction of Escherichia coli and Vibrio cholerae enterotoxins by an inhibitor of protein synthesis.

Authors:  M Levner; F P Wiener; B A Rubin
Journal:  Infect Immun       Date:  1977-01       Impact factor: 3.441

5.  A simple assay to detect Escherichia coli producing heat labile enterotoxin: results of a field study of the Biken tests in Bangladesh.

Authors:  T Honda; Q Akhtar; R I Glass; A K Kibriya
Journal:  Lancet       Date:  1981-09-19       Impact factor: 79.321

6.  Radial passive immune hemolysis assay for detection of heat-labile enterotoxin produced by individual colonies of Escherichia coli or Vibrio cholerae.

Authors:  M G Bramucci; R K Holmes
Journal:  J Clin Microbiol       Date:  1978-08       Impact factor: 5.948

7.  Enterotoxigenic Escherichia coli in central Canada.

Authors:  J Brunton; D Hinde; C Langston; R Gross; B Rowe; M Gurwith
Journal:  J Clin Microbiol       Date:  1980-04       Impact factor: 5.948

8.  Cyclic adenosine monophosphate and alteration of Chinese hamster ovary cell morphology: a rapid, sensitive in vitro assay for the enterotoxins of Vibrio cholerae and Escherichia coli.

Authors:  R L Guerrant; L L Brunton; T C Schnaitman; L I Rebhun; A G Gilman
Journal:  Infect Immun       Date:  1974-08       Impact factor: 3.441

9.  Rapid detection by a coagglutination test of heat-labile enterotoxin in cell lysates from blood agar-grown Escherichia coli.

Authors:  B Rönnberg; T Wadström
Journal:  J Clin Microbiol       Date:  1983-06       Impact factor: 5.948

10.  A two-year survey of the incidence of heat-labile enterotoxin-producing Escherichia coli and other enteric pathogens in travellers returning to the Sheffield area.

Authors:  P A Chapman; D L Mitchelmore
Journal:  Epidemiol Infect       Date:  1988-10       Impact factor: 2.451
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Review 2.  Diarrheagenic Escherichia coli.

Authors:  J P Nataro; J B Kaper
Journal:  Clin Microbiol Rev       Date:  1998-01       Impact factor: 26.132

3.  IS3 profiling identifies the enterohaemorrhagic Escherichia coli O-island 62 in a distinct enteroaggregative E. coli lineage.

Authors:  Iruka N Okeke; Louissa R Macfarlane-Smith; Jonathan N Fletcher; Anna M Snelling
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4.  Evaluation of non-radioactive trivalent DNA probe (LT, ST1a, ST1b) for detecting enterotoxigenic Escherichia coli.

Authors:  P A Chapman; C M Daly
Journal:  J Clin Pathol       Date:  1993-04       Impact factor: 3.411
  4 in total

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