Michelle V Eidt1, Fernanda Bordignon Nunes2, Leonardo Pedrazza1, Gabriela Caeran3, Giovana Pellegrin3, Denizar A S Melo1, Lia Possuelo3, Renan Trevisan Jost1, Henrique Bregolin Dias1, Márcio V F Donadio1, Jarbas R Oliveira4. 1. Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul, PUCRS, Porto Alegre, RS, Brazil. 2. Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul, PUCRS, Porto Alegre, RS, Brazil; Universidade Federal de Ciências da Saúde de Porto Alegre, UFCSPA, Porto Alegre, RS, Brazil. 3. Universidade de santa Cruz do Sul, UNISC, Santa Cruz do Sul, RS, Brazil. 4. Laboratório de Pesquisa em Biofísica Celular e Inflamação, Pontifícia Universidade Católica do Rio Grande do Sul, PUCRS, Porto Alegre, RS, Brazil. Electronic address: jarbas@pucrs.br.
Abstract
BACKGROUND: Sepsis is a major health care problem, with a significant mortality rate in intensive care units. We evaluated biochemical and inflammatory markers in patients with severe sepsis and septic shock and its association of with mortality rates. METHODS: Critically ill patients with diagnoses of sepsis - severe sepsis group (n=23) and septic shock group (n=25), and a control group (n=17) were recruited within 24h of entry into the ICU. Serum levels of inflammatory mediators were measured (IL-1β, IL-6, IL-8, IL-10, TNF-α, IL-18 and nitric oxide). We have also collected clinical parameters and laboratorial tests to estimate severity and organ dysfunction (APACHE II, SOFA, lactate). These results were compared between survivors and no survivors. RESULTS: IL-18 was directly related to mortality independently of other inflammatory mediators, especially IL-1β, although the inflammatory pathway is closely linked to inflammasome activation and both have simultaneous release in the infectious process. Mortality was directly proportional to IL-18 plasma levels, which did not occur with other inflammatory mediators. CONCLUSIONS: IL-18 is an important predictor of mortality in humans with both severe sepsis and septic shock, independent of IL-1β.
BACKGROUND:Sepsis is a major health care problem, with a significant mortality rate in intensive care units. We evaluated biochemical and inflammatory markers in patients with severe sepsis and septic shock and its association of with mortality rates. METHODS:Critically illpatients with diagnoses of sepsis - severe sepsis group (n=23) and septic shock group (n=25), and a control group (n=17) were recruited within 24h of entry into the ICU. Serum levels of inflammatory mediators were measured (IL-1β, IL-6, IL-8, IL-10, TNF-α, IL-18 and nitric oxide). We have also collected clinical parameters and laboratorial tests to estimate severity and organ dysfunction (APACHE II, SOFA, lactate). These results were compared between survivors and no survivors. RESULTS:IL-18 was directly related to mortality independently of other inflammatory mediators, especially IL-1β, although the inflammatory pathway is closely linked to inflammasome activation and both have simultaneous release in the infectious process. Mortality was directly proportional to IL-18 plasma levels, which did not occur with other inflammatory mediators. CONCLUSIONS:IL-18 is an important predictor of mortality in humans with both severe sepsis and septic shock, independent of IL-1β.
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Authors: Juan José Martínez-García; Helios Martínez-Banaclocha; Diego Angosto-Bazarra; Carlos de Torre-Minguela; Alberto Baroja-Mazo; Cristina Alarcón-Vila; Laura Martínez-Alarcón; Joaquín Amores-Iniesta; Fátima Martín-Sánchez; Giovanni A Ercole; Carlos M Martínez; Ada González-Lisorge; José Fernández-Pacheco; Piedad Martínez-Gil; Sahil Adriouch; Friedrich Koch-Nolte; Juan Luján; Francisco Acosta-Villegas; Pascual Parrilla; Carlos García-Palenciano; Pablo Pelegrin Journal: Nat Commun Date: 2019-06-20 Impact factor: 14.919
Authors: J Tallon; B Browning; F Couenne; C Bordes; F Venet; P Nony; F Gueyffier; V Moucadel; G Monneret; M Tayakout-Fayolle Journal: In Silico Biol Date: 2020