| Literature DB >> 26683181 |
Tomohiro Osaki1, Cai-Xia Wang1, Taro Tachibana1,2, Masayuki Azuma1, Masaya Kitamura1, Takeshi Nakanishi1.
Abstract
Overexpression of the epidermal growth factor receptor (EGFR) gene and dysregulation of EGFR signaling are observed in various cancer cells, and EGFR is a validated target for cancer therapy. In the present study, we report on the generation of two rat anti-EGFR antibodies (clones 2C2D3 and 4H7F4) by using the rat lymph node method. Flow cytometric analysis and immunofluorescence showed that both antibodies specifically bound to EGFR on the surface of cancer cells. Competitive analysis demonstrated that the epitope of each antibody had no overlap with that of the therapeutic anti-EGFR antibody cetuximab. These results suggest that 2C2D3 and 4H7F4 are potentially useful in EGFR-targeted cancer therapy.Entities:
Mesh：
Substances：
Year: 2015 PMID： 26683181 PMCID： PMC4685498 DOI： 10.1089/mab.2015.0034
Source DB: PubMed Journal: Monoclon Antib Immunodiagn Immunother ISSN： 2167-9436