BACKGROUND: High-throughput DNA sequencing has shown that the cutaneous microbiome varies due to different exogenous and endogenous factors. OBJECTIVES: To characterize the microbiome of cutaneous melanomas and melanocytic nevi. MATERIAL AND METHODS: Non-invasive swab specimens were taken from 15 cutaneous melanomas and 17 benign melanocytic nevi. Partial sequencing of the 16S ribosomal RNA gene was carried out on the 454 GS-FLX Titanium platform and the resulting sequence data was analysed by bioinformatics and statistical methods. 95% of the OTUs (Operational Taxonomic Units) belonged to four phyla: Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes. The genus Propionibacterium was overall the most common genus, followed by Staphylococcus and Corynebacterium. Statistical analysis showed no significant differences in the relative abundances of bacterial genera or bacterial diversity between the patient groups. Melanoma samples showed a marginally decreased cutaneous microbial diversity. CONCLUSION: Our data suggests that the skin microbiome may not be a useful diagnostic tool for melanoma and melanocytic nevi.
BACKGROUND: High-throughput DNA sequencing has shown that the cutaneous microbiome varies due to different exogenous and endogenous factors. OBJECTIVES: To characterize the microbiome of cutaneous melanomas and melanocytic nevi. MATERIAL AND METHODS: Non-invasive swab specimens were taken from 15 cutaneous melanomas and 17 benign melanocytic nevi. Partial sequencing of the 16S ribosomal RNA gene was carried out on the 454 GS-FLX Titanium platform and the resulting sequence data was analysed by bioinformatics and statistical methods. 95% of the OTUs (Operational Taxonomic Units) belonged to four phyla: Firmicutes, Actinobacteria, Proteobacteria and Bacteroidetes. The genus Propionibacterium was overall the most common genus, followed by Staphylococcus and Corynebacterium. Statistical analysis showed no significant differences in the relative abundances of bacterial genera or bacterial diversity between the patient groups. Melanoma samples showed a marginally decreased cutaneous microbial diversity. CONCLUSION: Our data suggests that the skin microbiome may not be a useful diagnostic tool for melanoma and melanocytic nevi.
Authors: Priyanka Kumar; Danielle Brazel; Julia DeRogatis; Jennifer B Goldstein Valerin; Katrine Whiteson; Warren A Chow; Roberto Tinoco; Justin T Moyers Journal: Cancer Metastasis Rev Date: 2022-04-27 Impact factor: 9.237
Authors: Marna E Ericson; Edward B Breitschwerdt; Paul Reicherter; Cole Maxwell; Ricardo G Maggi; Richard G Melvin; Azar H Maluki; Julie M Bradley; Jennifer C Miller; Glenn E Simmons; Jamie Dencklau; Keaton Joppru; Jack Peterson; Will Bae; Janet Scanlon; Lynne T Bemis Journal: Pathogens Date: 2021-03-10