INTRODUCTION: This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. METHODS: Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. RESULTS: PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. CONCLUSIONS: Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.
INTRODUCTION: This study describes the occurrence of trypanosomatids in phlebotomines in Brasília, Brazil. METHODS: Two hundred and ten females of 13 sand fly species were analyzed by polymerase chain reaction (PCR) using different molecular markers (D7 24Sα rRNA, kDNA, and ITS1) and sequencing. RESULTS: PCR revealed trypanosomatid-positive samples from Nyssomyia whitmani and Evandromyia evandroi, which were negative by kDNA and ITS1 Leishmania-specific PCRs. DNA sequence analysis of D7 24Sα rRNA amplicons indicated the occurrence of Blastocrithidia sp. and Trypanosoma sp. in Nyssomyia whitmani and Evandromyia evandroi, respectively. CONCLUSIONS: Two trypanosomatid species other than Leishmania sp. were found to circulate in sand flies in Central Brazil.
Authors: Luz H Patiño; Adriana C Castillo-Castañeda; Marina Muñoz; Jesus E Jaimes; Nicolas Luna-Niño; Carolina Hernández; Martha S Ayala; Patricia Fuya; Claudia Mendez; Carlos E Hernández-Pereira; Lourdes Delgado; Claudia M Sandoval-Ramírez; Plutarco Urbano; Alberto Paniz-Mondolfi; Juan David Ramírez Journal: Microbiol Spectr Date: 2021-10-13
Authors: Filipe C Reis; Thaís T C Minuzzi-Souza; Mariana Neiva; Renata V Timbó; Igor O B de Morais; Thiago M de Lima; Mariana Hecht; Nadjar Nitz; Rodrigo Gurgel-Gonçalves Journal: Vet Med Sci Date: 2019-11-19