K S Roopa1, K Karthika2, Madhan Sugumar3, Chanaveerappa Bammigatti4, Suryanarayana Bettadpura Shamanna5, Belgode Narasimha Harish6. 1. PhD scholar, Department of Microbiology, Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry, India . 2. Senior Research Fellow, Department of Microbiology, Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry, India . 3. Senior Research Fellow, Department of Microbiology, Jawaharlal Institute of Post Graduate, Medical Education and Research , Puducherry, India . 4. Associate Professor, Department of Medicine, Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry, India . 5. Associate Professor, Departmentof Medicine, Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry, India . 6. Senior Professor and Head, Department of Microbiology, Jawaharlal Institute of Post Graduate Medical Education and Research , Puducherry, India .
Abstract
INTRODUCTION: Scrub typhus, a zoonotic disease is one of the most covert emerging and re-emerging Rickettsial infections. There is an upsurge in the incidence of the disease worldwide with ever-changing habitat. Clinical diagnosis of scrub typhus is challenging as the signs and symptoms of scrub typhus are similar to other febrile illnesses. In developing countries, among the various laboratory tests to diagnose scrub typhus, Weil-Felix test is commonly performed despite its low sensitivity. The Immunofluorescence antibody (IFA) test has its limitations in terms of cost and expertise required. The present study was conducted to determine the seropositivity of IgM ELISA for scrub typhus in clinically suspected cases. MATERIALS AND METHODS: Weil-Felix test and IgM ELISA were performed using clinically suspected cases of scrub typhus using commercially available kits. RESULTS: Out of 482 samples tested, 109 were positive by both Weil-Felix test and IgM ELISA. One hundred and sixteen samples which were negative by Weil-Felix test reacted positive by IgM ELISA. Fourteen samples which were positive by Weil-Felix test were negative by ELISA. CONCLUSION: Owing to the limitations of the Weil-Felix test and IFA, commercially available recombinant IgM ELISA which has a good sensitivity and specificity may be an alternative in laboratories with moderate set up.
INTRODUCTION:Scrub typhus, a zoonotic disease is one of the most covert emerging and re-emerging Rickettsial infections. There is an upsurge in the incidence of the disease worldwide with ever-changing habitat. Clinical diagnosis of scrub typhus is challenging as the signs and symptoms of scrub typhus are similar to other febrile illnesses. In developing countries, among the various laboratory tests to diagnose scrub typhus, Weil-Felix test is commonly performed despite its low sensitivity. The Immunofluorescence antibody (IFA) test has its limitations in terms of cost and expertise required. The present study was conducted to determine the seropositivity of IgM ELISA for scrub typhus in clinically suspected cases. MATERIALS AND METHODS: Weil-Felix test and IgM ELISA were performed using clinically suspected cases of scrub typhus using commercially available kits. RESULTS: Out of 482 samples tested, 109 were positive by both Weil-Felix test and IgM ELISA. One hundred and sixteen samples which were negative by Weil-Felix test reacted positive by IgM ELISA. Fourteen samples which were positive by Weil-Felix test were negative by ELISA. CONCLUSION: Owing to the limitations of the Weil-Felix test and IFA, commercially available recombinant IgM ELISA which has a good sensitivity and specificity may be an alternative in laboratories with moderate set up.
Entities:
Keywords:
IgM ELISA; Orientia tsutsugamushi; Weil-Felix test
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