Caroline Desclée de Maredsous1, Raish Oozeer2, Pierre Barbillon3, Tristan Mary-Huard4, Corine Delteil5, François Blachier5, Daniel Tomé5, Eline M van der Beek2, Anne-Marie Davila6. 1. UMR 914 Nutrition Physiology and Ingestive Behavior, French National Institute for Agricultural Research (INRA)/AgroParisTech, Paris Saclay University, Paris, France; Danone Nutricia Research, Utrecht, Netherlands; 2. Danone Nutricia Research, Utrecht, Netherlands; 3. UMR 518 Applied Mathematics and Informatics (MIA), French National Institute for Agricultural Research (INRA)/AgroParisTech, Paris Saclay University, Paris, France; and. 4. UMR 518 Applied Mathematics and Informatics (MIA), French National Institute for Agricultural Research (INRA)/AgroParisTech, Paris Saclay University, Paris, France; and Quantitative Genetics Evolution Le Moulon, French National Institute for Agricultural Research (INRA), Paris-Sud University, Paris Saclay University, AgroParisTech, CNRS, Gif-sur-Yvette, France. 5. UMR 914 Nutrition Physiology and Ingestive Behavior, French National Institute for Agricultural Research (INRA)/AgroParisTech, Paris Saclay University, Paris, France; 6. UMR 914 Nutrition Physiology and Ingestive Behavior, French National Institute for Agricultural Research (INRA)/AgroParisTech, Paris Saclay University, Paris, France; davila@agroparistech.fr.
Abstract
BACKGROUND: Early-life nutrition has a programming effect on later metabolic health; however, the impact of exposure to a high-protein (HP) diet is still being investigated. OBJECTIVE: This study evaluated the consequences on pup phenotype of an HP diet during gestation and lactation and after weaning. METHODS: Wistar rat dams were separated into 2 groups fed an HP (55% protein) or normal protein (NP) (control; 20% protein) isocaloric diet during gestation, and each group subsequently was separated into 2 subgroups that were fed an HP or NP diet during lactation. After weaning, male and female pups from each mother subgroup were separated into 2 groups that were fed either an NP or HP diet until they were 6 wk old. Measurements included weight, food intake, body composition, blood glucose, insulin, glucagon, leptin, insulin-like growth factor I, and lipids. RESULTS: Feeding mothers the HP diet during gestation or lactation induced lower postweaning pup weight (gestation diet × time, P < 0.0001; lactation diet × time, P < 0.0001). Regardless of dams' diets, pups receiving HP compared with NP diet after weaning had 7% lower weight (NP, 135.0 ± 2.6 g; HP, 124.4 ± 2.5 g; P < 0.0001), 16% lower total energy intake (NP, 777 ± 14 kcal; HP, 649 ± 13 kcal; P < 0.0001) and 31% lower adiposity (P < 0.0001). Pups receiving HP compared with NP diet after weaning had increased blood glucose, insulin, and glucagon when food deprived (P < 0.0001 for all). The HP compared with the NP diet during gestation induced higher blood glucose in food-deprived rats (NP, 83.2 ± 2.1 mg/dL; HP, 91.2 ± 2.1 mg/dL; P = 0.046) and increased plasma insulin in fed pups receiving the postweaning NP diet (gestation diet × postweaning diet, P = 0.02). CONCLUSION: Increasing the protein concentration of the rat dams' diet during gestation, and to a lesser extent during lactation, and of the pups' diet after weaning influenced pup phenotype, including body weight, fat accumulation, food intake, and glucose tolerance at 6 wk of age.
BACKGROUND: Early-life nutrition has a programming effect on later metabolic health; however, the impact of exposure to a high-protein (HP) diet is still being investigated. OBJECTIVE: This study evaluated the consequences on pup phenotype of an HP diet during gestation and lactation and after weaning. METHODS: Wistar rat dams were separated into 2 groups fed an HP (55% protein) or normal protein (NP) (control; 20% protein) isocaloric diet during gestation, and each group subsequently was separated into 2 subgroups that were fed an HP or NP diet during lactation. After weaning, male and female pups from each mother subgroup were separated into 2 groups that were fed either an NP or HP diet until they were 6 wk old. Measurements included weight, food intake, body composition, blood glucose, insulin, glucagon, leptin, insulin-like growth factor I, and lipids. RESULTS: Feeding mothers the HP diet during gestation or lactation induced lower postweaning pup weight (gestation diet × time, P < 0.0001; lactation diet × time, P < 0.0001). Regardless of dams' diets, pups receiving HP compared with NP diet after weaning had 7% lower weight (NP, 135.0 ± 2.6 g; HP, 124.4 ± 2.5 g; P < 0.0001), 16% lower total energy intake (NP, 777 ± 14 kcal; HP, 649 ± 13 kcal; P < 0.0001) and 31% lower adiposity (P < 0.0001). Pups receiving HP compared with NP diet after weaning had increased blood glucose, insulin, and glucagon when food deprived (P < 0.0001 for all). The HP compared with the NP diet during gestation induced higher blood glucose in food-deprived rats (NP, 83.2 ± 2.1 mg/dL; HP, 91.2 ± 2.1 mg/dL; P = 0.046) and increased plasma insulin in fed pups receiving the postweaning NP diet (gestation diet × postweaning diet, P = 0.02). CONCLUSION: Increasing the protein concentration of the rat dams' diet during gestation, and to a lesser extent during lactation, and of the pups' diet after weaning influenced pup phenotype, including body weight, fat accumulation, food intake, and glucose tolerance at 6 wk of age.
Authors: Peter K Ajuogu; Mitchell Wolden; James R McFarlane; Robert A Hart; Debra J Carlson; Tom Van der Touw; Neil A Smart Journal: J Anim Sci Date: 2020-01-01 Impact factor: 3.159
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