Rachid Y Yahiaoui1,2, Casper D J den Heijer1, Petra Wolfs1, Cathrien A Bruggeman1, Ellen E Stobberingh1,3. 1. Departement of Medical Microbiology, Maastricht University Medical Centre, Maastricht, The Netherlands. 2. Department of Medical Microbiology, Haga Hospital, The Hague, The Netherlands. 3. National Institute for Public Health & the Environment, RIVM, Bilthoven, The Netherlands.
Abstract
AIM: The objective of this study is to compare various Streptococcus pneumoniae identification methods. MATERIALS & METHODS: In total, 1371 putative S. pneumoniae isolates were tested with three phenotypic methods and a molecular-based method targeting a virulence factor (CpsA). We assessed the sensitivity and the specificity of each method and widely used S. pneumoniae identification algorithm. RESULTS: None of the methods or the identification algorithm used separately was able to correctly identify all S. pneumoniae isolates. Furthermore, a high rate of optochin resistance was found. CONCLUSIONS: We demonstrated the failure of the current S. pneumoniae identification methods and optochin susceptibility-based algorithm. In addition, the high rate of optochin resistance might justify the necessity of a close monitoring of optochin susceptibility.
AIM: The objective of this study is to compare various Streptococcus pneumoniae identification methods. MATERIALS & METHODS: In total, 1371 putative S. pneumoniae isolates were tested with three phenotypic methods and a molecular-based method targeting a virulence factor (CpsA). We assessed the sensitivity and the specificity of each method and widely used S. pneumoniae identification algorithm. RESULTS: None of the methods or the identification algorithm used separately was able to correctly identify all S. pneumoniae isolates. Furthermore, a high rate of optochin resistance was found. CONCLUSIONS: We demonstrated the failure of the current S. pneumoniae identification methods and optochin susceptibility-based algorithm. In addition, the high rate of optochin resistance might justify the necessity of a close monitoring of optochin susceptibility.
Entities:
Keywords:
PCR; Streptococcus pneumoniae; bile solubility; colony morphology; identification; optochin
Authors: Anne L Wyllie; Yvonne Pannekoek; Sandra Bovenkerk; Jody van Engelsdorp Gastelaars; Bart Ferwerda; Diederik van de Beek; Elisabeth A M Sanders; Krzysztof Trzciński; Arie van der Ende Journal: Open Biol Date: 2017-09 Impact factor: 6.411
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