Karin E Elberse1, Gertjan H J Wagenvoort2, Gerlinde N Pluister1, Hester E de Melker1, Elisabeth A M Sanders1,3, Arie van der Ende4, Mirjam J Knol1. 1. Centre for Infectious Disease Control Netherlands (CIb), National Institute for Public Health & the Environment (RIVM), Bilthoven, The Netherlands. 2. Department of Medical Microbiology & Immunology, St. Antonius Hospital, Nieuwegein, The Netherlands. 3. Department of Immunology & Infectious Diseases, University Medical Center Utrecht, Wilhelmina Children's Hospital, Utrecht, The Netherlands. 4. Department of Medical Microbiology, The Netherlands Reference Laboratory for Bacterial Meningitis, Academic Medical Center, Amsterdam, The Netherlands.
Abstract
BACKGROUND: Vaccination of infants with pneumococcal conjugate vaccines (PCV) has resulted in major shifts in circulating serotypes. AIM: To investigate the impact of PCV7 on the clonal composition of the pneumococcal population, and the relation of clonal lineages and clinical outcome. MATERIALS & METHODS: By using multiple-locus variable number of tandem repeat analysis, we assessed the pneumococcal populations before (n = 1154), 2-3 years after (n = 1190) and 4-6 years after (n = 1244) the introduction of PCV7 in The Netherlands. RESULTS: We found statistically significant shifts in clonal lineages within serotypes 1 and 12F based on multiple-locus variable number of tandem repeat analysis results after the implementation of PCV7. Within serotype 12F, the increasing clonal lineage was significantly more associated with pneumonia. CONCLUSION: Shifts in clonal lineages within serotypes could impact the outcomes of pneumococcal disease and fill the niche of the vaccine serotypes.
BACKGROUND: Vaccination of infants with pneumococcal conjugate vaccines (PCV) has resulted in major shifts in circulating serotypes. AIM: To investigate the impact of PCV7 on the clonal composition of the pneumococcal population, and the relation of clonal lineages and clinical outcome. MATERIALS & METHODS: By using multiple-locus variable number of tandem repeat analysis, we assessed the pneumococcal populations before (n = 1154), 2-3 years after (n = 1190) and 4-6 years after (n = 1244) the introduction of PCV7 in The Netherlands. RESULTS: We found statistically significant shifts in clonal lineages within serotypes 1 and 12F based on multiple-locus variable number of tandem repeat analysis results after the implementation of PCV7. Within serotype 12F, the increasing clonal lineage was significantly more associated with pneumonia. CONCLUSION: Shifts in clonal lineages within serotypes could impact the outcomes of pneumococcal disease and fill the niche of the vaccine serotypes.
Authors: Rebecca A Gladstone; Vanessa Devine; Jessica Jones; David Cleary; Johanna M Jefferies; Stephen D Bentley; Saul N Faust; Stuart C Clarke Journal: Microb Genom Date: 2017-06-09