| Literature DB >> 26673640 |
Adilah Bahadoor1, Danielle Schneiderman1, Larissa Gemmill1, Whynn Bosnich1, Barbara Blackwell1, Jeremy E Melanson2, Garnet McRae2, Linda J Harris1.
Abstract
A second structural gene required for culmorin biosynthesis in the plant pathogen Fusarium graminearum is described. Clm2 encodes a regio- and stereoselective cytochrome P450 monooxygenase for C-11 of longiborneol (1). Clm2 gene disruptants were grown in liquid culture and assessed for culmorin production via HPLC-evaporative light scattering detection. The analysis indicated a complete loss of culmorin (2) from the liquid culture of the ΔClm2 mutants. Culmorin production resumed in a ΔClm2 complementation experiment. A detailed analysis of the secondary metabolites extracted from the large-scale liquid culture of disruptant ΔClm2D20 revealed five new natural products: 3-hydroxylongiborneol (3), 5-hydroxylongiborneol (4), 12-hydroxylongiborneol (5), 15-hydroxylongiborneol (6), and 11-epi-acetylculmorin (7). The structures of the new compounds were elucidated by a combination of HRMS, 1D and 2D NMR, and X-ray crystallography.Entities:
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Year: 2015 PMID： 26673640 DOI： 10.1021/acs.jnatprod.5b00676
Source DB: PubMed Journal: J Nat Prod ISSN： 0163-3864 Impact factor: 4.050