Achraf Al Faraj1, Asma Sultana Shaik2, Baraa Al Sayed1, Rabih Halwani3, Ibrahim Al Jammaz4. 1. Department of Radiological Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi Arabia. 2. Prince Naif Health Research Center, King Saud University, Riyadh 12372, Saudi Arabia. 3. Prince Naif Center for Immunology Research & Asthma Research Chair, Pediatrics Department, College of Medicine, King Saud University, Riyadh 12372, Saudi Arabia. 4. Cyclotron & Radiopharmaceutical Department, King Faisal Specialist Hospital & Research Centre, Riyadh 12713, Saudi Arabia.
Abstract
BACKGROUND: The limitation of current breast cancer treatments was elucidated by the presence of cancer stem cells (CSCs) that play essential role in cancer initiation, progression, resistance, recurrence and metastasis. Materials & methods: Biocompatible multimodality single-walled carbon nanotube (SWCNT) nanoprobes were developed. The biodistribution and preferential homing of CD44 antibody-conjugated SWCNTs toward the tumor site were monitored using MRI, single-photon emission computed tomography and near-infrared fluorescence imaging noninvasive imaging modalities. RESULTS: Quantification of SWCNTs by sensitively measuring iron content in sorted CSC populations using inductively coupled plasma-mass spectrometry confirmed the enhanced selective targeting of anti-CD44 SWCNT and immunohistochemistry analyses revealed enhanced colocalization with areas rich in CD44 receptors. DISCUSSION & CONCLUSION: This preclinical study provided encouraging results for efficient targeting of breast CSCs and perspectives for further clinical studies to confirm the efficacy and safety of the designed nanocarriers.
BACKGROUND: The limitation of current breast cancer treatments was elucidated by the presence of cancer stem cells (CSCs) that play essential role in cancer initiation, progression, resistance, recurrence and metastasis. Materials & methods: Biocompatible multimodality single-walled carbon nanotube (SWCNT) nanoprobes were developed. The biodistribution and preferential homing of CD44 antibody-conjugated SWCNTs toward the tumor site were monitored using MRI, single-photon emission computed tomography and near-infrared fluorescence imaging noninvasive imaging modalities. RESULTS: Quantification of SWCNTs by sensitively measuring iron content in sorted CSC populations using inductively coupled plasma-mass spectrometry confirmed the enhanced selective targeting of anti-CD44 SWCNT and immunohistochemistry analyses revealed enhanced colocalization with areas rich in CD44 receptors. DISCUSSION & CONCLUSION: This preclinical study provided encouraging results for efficient targeting of breast CSCs and perspectives for further clinical studies to confirm the efficacy and safety of the designed nanocarriers.
Entities:
Keywords:
MRI; SPECT; biodistribution; breast cancer; cancer stem cells; carbon nanotubes; nanoparticles; noninvasive imaging; targeting