Noriko Yoneda1, Satoshi Yoneda1, Hideki Niimi2, Tomohiro Ueno2, Shirou Hayashi2, Mika Ito1, Arihiro Shiozaki1, Daichi Urushiyama3,4, Kenichiro Hata3, Wataru Suda5, Masahira Hattori5,6, Mika Kigawa7, Isao Kitajima2, Shigeru Saito1. 1. Department of Obstetrics and Gynecology, University of Toyama, Toyama, Japan. 2. Clinical Laboratory Center, Toyama University Hospital, Toyama, Japan. 3. Department of Maternal-Fetal Biology, National Research Institute for Child Health and Development, Tokyo, Japan. 4. Department of Obstetrics and Gynecology, Faculty of Medicine, Fukuoka University, Fukuoka, Japan. 5. Laboratory of Metagenomics, Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Japan. 6. Graduate School of Advanced Science and Engineering, Waseda University, Tokyo, Japan. 7. Department of Public Health Faculty of Medicine, University of Toyama, Toyama, Japan.
Abstract
PROBLEM: To study the relationship between perinatal prognosis in cases of preterm labor (PTL) and polymicrobial infection in amniotic fluid (AF) and intra-amniotic (IA) inflammation using a highly sensitive and reliable PCR-based method. METHOD OF STUDY: To detect prokaryotes using a nested PCR-based method, eukaryote-made thermostable DNA polymerase without bacterial DNA contamination was used in combination with bacterial universal primers. We collected AF aseptically from 118 PTL cases and 50 term subjects. RESULTS: The prevalence of microorganisms was 33% (39/118) by PCR and only 7.6% (9/118) by culture. PTL caused by a combination of positive Mycoplasma/Ureaplasma and other bacteria had significantly higher AF IL-8 levels and a significantly shorter amniocentesis-to-delivery interval. CONCLUSIONS: Our newly established PCR method is useful for detecting IA microorganisms. Polymicrobial infection with Mycoplasma/Ureaplasma and other bacteria induces severe IA inflammation associated with poor perinatal prognosis in PTL.
PROBLEM: To study the relationship between perinatal prognosis in cases of preterm labor (PTL) and polymicrobial infection in amniotic fluid (AF) and intra-amniotic (IA) inflammation using a highly sensitive and reliable PCR-based method. METHOD OF STUDY: To detect prokaryotes using a nested PCR-based method, eukaryote-made thermostable DNA polymerase without bacterial DNA contamination was used in combination with bacterial universal primers. We collected AF aseptically from 118 PTL cases and 50 term subjects. RESULTS: The prevalence of microorganisms was 33% (39/118) by PCR and only 7.6% (9/118) by culture. PTL caused by a combination of positive Mycoplasma/Ureaplasma and other bacteria had significantly higher AFIL-8 levels and a significantly shorter amniocentesis-to-delivery interval. CONCLUSIONS: Our newly established PCR method is useful for detecting IA microorganisms. Polymicrobial infection with Mycoplasma/Ureaplasma and other bacteria induces severe IA inflammation associated with poor perinatal prognosis in PTL.
Authors: Julie A Potter; Mancy Tong; Paulomi Aldo; Ja Young Kwon; Mary Pitruzzello; Gil Mor; Vikki M Abrahams Journal: J Reprod Immunol Date: 2020-04-08 Impact factor: 4.054