Natasa Strbo1, Maria L Alcaide2, Laura Romero1, Hector Bolivar2, Deborah Jones3, Eckhard R Podack1, Margaret A Fischl2. 1. Department of Microbiology and Immunology, University of Miami Miller School of Medicine, Miami, FL, USA. 2. Division of Infectious Diseases, University of Miami Miller School of Medicine, Miami, FL, USA. 3. Department of Psychiatry and Behavioral Sciences, University of Miami Miller School of Medicine, Miami, FL, USA.
Abstract
PROBLEM: Human gamma delta (GD) T cells play a well-documented role in epithelial barrier surveillance and protection. Two subsets of GD T cells, defined by the use of either the Vdelta2 (GD2) or Vdelta1 (GD1) TCR, predominate. We hypothesized that endocervical GD T cells play important role in lower genital tract anti-HIV immune responses. METHOD OF STUDY: HIV-infected (n = 18) and HIV-uninfected (n = 19) pre-menopausal women participating in the WIHS cohort were recruited. Frequency and phenotype of GD T cells were determined in endocervical cytobrush samples and peripheral blood by multicolor flow cytometry. RESULTS: We found depletion of GD2 cells in the blood of HIV-infected women as well as significant decrease in the frequency of endocervical GD1 cells compared to uninfected women. CONCLUSION: We report for the first time, the GD1 cells are a predominant endocervical T-cell subset that is significantly decreased in HIV-infected women.
PROBLEM: Human gamma delta (GD) T cells play a well-documented role in epithelial barrier surveillance and protection. Two subsets of GD T cells, defined by the use of either the Vdelta2 (GD2) or Vdelta1 (GD1) TCR, predominate. We hypothesized that endocervical GD T cells play important role in lower genital tract anti-HIV immune responses. METHOD OF STUDY: HIV-infected (n = 18) and HIV-uninfected (n = 19) pre-menopausal women participating in the WIHS cohort were recruited. Frequency and phenotype of GD T cells were determined in endocervical cytobrush samples and peripheral blood by multicolor flow cytometry. RESULTS: We found depletion of GD2 cells in the blood of HIV-infectedwomen as well as significant decrease in the frequency of endocervical GD1 cells compared to uninfected women. CONCLUSION: We report for the first time, the GD1 cells are a predominant endocervical T-cell subset that is significantly decreased in HIV-infectedwomen.
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