INTRODUCTION: A previous study showed the unique character of Nigerian red propolis from Rivers State, Nigeria (RSN), with regards to chemical composition and activity against Trypanosoma brucei in comparison with other African propolis. OBJECTIVE: To carry out fractionation and biological testing of Nigerian propolis in order to isolate compounds with anti-trypanosomal activity. To compare the composition of the RSN propolis with the composition of Brazilian red propolis. METHODOLOGY: Profiling was carried out using HPLC-UV-ELSD and HPLC-Orbitrap-FTMS on extracts of two samples collected from RSN with data extraction using MZmine software. Isolation was carried out by normal phase and reversed phase MPLC. Elucidation of the compounds with a purity > 95% was performed by 1D/2D NMR HRMS and HRLC-MS(n) . RESULTS: Ten phenolic compounds were isolated or in the case of liquiritigenin partially purified. Data for nine of these correlated with literature reports of known compounds i.e. one isoflavanone, calycosin (1); two flavanones, liquiritigenin (2) and pinocembrin (5); an isoflavan, vestitol (3); a pterocarpan, medicarpin (4); two prenylflavanones, 8-prenylnaringenin (7) and 6-prenylnaringenin (8); and two geranyl flavonoids, propolin D (9) and macarangin (10). The tenth was elucidated as a previously undescribed dihydrobenzofuran (6). The isolated compounds were tested against Trypanosoma brucei and displayed moderate to high activity. Some of the compounds tested had similar activity against wild type T. brucei and two strains displaying pentamidine resistance. CONCLUSION: Nigerian propolis from RSN has some similarities with Brazilian red propolis. The propolis displayed anti-trypanosomal activity at a potentially useful level.
INTRODUCTION: A previous study showed the unique character of Nigerian red propolis from Rivers State, Nigeria (RSN), with regards to chemical composition and activity against Trypanosoma brucei in comparison with other African propolis. OBJECTIVE: To carry out fractionation and biological testing of Nigerian propolis in order to isolate compounds with anti-trypanosomal activity. To compare the composition of the RSN propolis with the composition of Brazilian red propolis. METHODOLOGY: Profiling was carried out using HPLC-UV-ELSD and HPLC-Orbitrap-FTMS on extracts of two samples collected from RSN with data extraction using MZmine software. Isolation was carried out by normal phase and reversed phase MPLC. Elucidation of the compounds with a purity > 95% was performed by 1D/2D NMR HRMS and HRLC-MS(n) . RESULTS: Ten phenolic compounds were isolated or in the case of liquiritigenin partially purified. Data for nine of these correlated with literature reports of known compounds i.e. one isoflavanone, calycosin (1); two flavanones, liquiritigenin (2) and pinocembrin (5); an isoflavan, vestitol (3); a pterocarpan, medicarpin (4); two prenylflavanones, 8-prenylnaringenin (7) and 6-prenylnaringenin (8); and two geranyl flavonoids, propolin D (9) and macarangin (10). The tenth was elucidated as a previously undescribed dihydrobenzofuran (6). The isolated compounds were tested against Trypanosoma brucei and displayed moderate to high activity. Some of the compounds tested had similar activity against wild type T. brucei and two strains displaying pentamidine resistance. CONCLUSION: Nigerian propolis from RSN has some similarities with Brazilian red propolis. The propolis displayed anti-trypanosomal activity at a potentially useful level.
Authors: Yasser A Elnakady; Ahmed I Rushdi; Raimo Franke; Nael Abutaha; Hossam Ebaid; Mohannad Baabbad; Mohamed O M Omar; Ahmad A Al Ghamdi Journal: Sci Rep Date: 2017-02-06 Impact factor: 4.379
Authors: Ruwida Omar; John O Igoli; Tong Zhang; Alexander I Gray; Godwin U Ebiloma; Carol J Clements; James Fearnley; RuAngeli Edrada Ebel; Tim Paget; Harry P de Koning; David G Watson Journal: Sci Rep Date: 2017-04-19 Impact factor: 4.379
Authors: Adullah Alotaibi; Godwin U Ebiloma; Roderick Williams; Ibrahim A Alfayez; Manal J Natto; Sameah Alenezi; Weam Siheri; Malik AlQarni; John O Igoli; James Fearnley; Harry P De Koning; David G Watson Journal: Molecules Date: 2021-06-26 Impact factor: 4.411
Authors: Weam Siheri; Tong Zhang; Godwin Unekwuojo Ebiloma; Marco Biddau; Nicola Woods; Muattaz Yassein Hussain; Carol J Clements; James Fearnley; RuAngelie Edrada Ebel; Timothy Paget; Sylke Muller; Katharine C Carter; Valerie A Ferro; Harry P De Koning; David G Watson Journal: PLoS One Date: 2016-05-19 Impact factor: 3.240
Authors: Abdullah Alotaibi; Godwin U Ebiloma; Roderick Williams; Samya Alenezi; Anne-Marie Donachie; Selome Guillaume; John O Igoli; James Fearnley; Harry P de Koning; David G Watson Journal: Sci Rep Date: 2019-08-06 Impact factor: 4.379