R Ladurner1, K Hallfeldt2, N Al Arabi2, J Gallwas3, U Mortensen4, S Sommerey2. 1. Klinik für Allgemeine, Viszeral-, Transplantations-, Gefäß- und Thoraxchirurgie, Klinikum der Universität München, Campus Innenstadt, Nußbaumstr. 20, 80336, München, Deutschland. Roland.Ladurner@med.uni-muenchen.de. 2. Klinik für Allgemeine, Viszeral-, Transplantations-, Gefäß- und Thoraxchirurgie, Klinikum der Universität München, Campus Innenstadt, Nußbaumstr. 20, 80336, München, Deutschland. 3. Klinik und Poliklinik für Frauenheilkunde und Geburtshilfe, Klinikum der Universität München, Campus Großhadern, Marchioninistr. 15, 81377, München, Deutschland. 4. Institut für Psychologie, Westfälische Wilhelms-Universität, Fliednerstr. 21, 48149, Münster, Deutschland.
Abstract
BACKGROUND: Optical coherence tomography (OCT) is a high-resolution imaging technique that allows the identification of microarchitectural features in real-time. OBJECTIVE: Can OCT be used to differentiate parathyroid tissue from other cervical tissue entities? MATERIAL AND METHODS: All investigations were carried out during cervical operations. Initially, ex vivo images were analyzed to define morphological imaging criteria for each tissue entity. These criteria were used to evaluate a first series of ex vivo images. In a second phase the practicability of the technique was investigated in vivo and in the third phase backscattering intensity measurements were analyzed employing linear discriminant analysis (LDA). RESULTS: In the ex vivo series parathyroid tissue could be differentiated from other tissue entities with a sensitivity and specificity of 84 % and 94 %, respectively. Parathyroid tissue was correctly identified in the in vivo series in only 69.2 %. The analysis of backscattering intensity profiles employing LDA reliably distinguished between the different tissue types. CONCLUSION: The OCT images displayed typical characteristics for each tissue entity. Due to technical problems in handling the probe the in vivo OCT images were of much poorer quality. Backscattering intensity measurements illustrated that OCT images provide an individual profile for each tissue entity independent of the defined morphological assessment criteria. The results show that OCT is fundamentally suitable for intraoperative differentiation of tissues.
BACKGROUND: Optical coherence tomography (OCT) is a high-resolution imaging technique that allows the identification of microarchitectural features in real-time. OBJECTIVE: Can OCT be used to differentiate parathyroid tissue from other cervical tissue entities? MATERIAL AND METHODS: All investigations were carried out during cervical operations. Initially, ex vivo images were analyzed to define morphological imaging criteria for each tissue entity. These criteria were used to evaluate a first series of ex vivo images. In a second phase the practicability of the technique was investigated in vivo and in the third phase backscattering intensity measurements were analyzed employing linear discriminant analysis (LDA). RESULTS: In the ex vivo series parathyroid tissue could be differentiated from other tissue entities with a sensitivity and specificity of 84 % and 94 %, respectively. Parathyroid tissue was correctly identified in the in vivo series in only 69.2 %. The analysis of backscattering intensity profiles employing LDA reliably distinguished between the different tissue types. CONCLUSION: The OCT images displayed typical characteristics for each tissue entity. Due to technical problems in handling the probe the in vivo OCT images were of much poorer quality. Backscattering intensity measurements illustrated that OCT images provide an individual profile for each tissue entity independent of the defined morphological assessment criteria. The results show that OCT is fundamentally suitable for intraoperative differentiation of tissues.
Authors: Roland Ladurner; Klaus K J Hallfeldt; Norah Al Arabi; Herbert Stepp; Susanna Mueller; Julia K S Gallwas Journal: Lasers Surg Med Date: 2013-11-19 Impact factor: 4.025
Authors: Sandra Sommerey; Roland Ladurner; Norah Al Arabi; Uwe Mortensen; Klaus Hallfeldt; Julia Gallwas Journal: Lasers Surg Med Date: 2015-05-29 Impact factor: 4.025
Authors: Luiz C Conti de Freitas; Eimear Phelan; Linbo Liu; Joseph Gardecki; Eman Namati; Willian C Warger; Guillermo J Tearney; Gregory W Randolph Journal: Head Neck Date: 2013-11-21 Impact factor: 3.147
Authors: Julia K S Gallwas; Lisa Turk; Herbert Stepp; Susanna Mueller; Robert Ochsenkuehn; Klaus Friese; Christian Dannecker Journal: Lasers Surg Med Date: 2011-03 Impact factor: 4.025
Authors: Mayret M Castillo; Graham Mowatt; Andrew Elders; Noemi Lois; Cynthia Fraser; Rodolfo Hernández; Winfried Amoaku; Jennifer M Burr; Andrew Lotery; Craig R Ramsay; Augusto Azuara-Blanco Journal: Ophthalmology Date: 2014-10-22 Impact factor: 12.079