A W B Reyes1,2, D G Kim1, H L T Simborio1, H T Hop1, L T Arayan1, W Min1, J J Lee3, H H Chang4, S Kim1,4. 1. Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju, Korea. 2. Department of Veterinary Paraclinical Sciences, College of Veterinary Medicine, University of the Philippines Los Baños, Laguna, Philippines. 3. Animal and Plant Quarantine Agency, Anyang, Gyeonggi-do, Korea. 4. Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Korea.
Abstract
AIMS: To investigate the effects of methyl gallate (MG) on murine macrophages, cytokine production and treatment of Brucella abortus infection using a mouse model. METHODS AND RESULTS: MG-treated cells displayed increased F-actin polymerization and modest increase in ERK, JNK and p38α phosphorylation levels. The mice were intraperitoneally infected with Br. abortus and were orally treated with PBS or MG for 14 days. The weight and bacterial number from each spleen were monitored, and the serum was evaluated for cytokine production. The spleen proliferation and bacterial burden were lower in the MG-treated group than in the MG-untreated control. The noninfected MG-treated mice displayed increased production of TNF, IFN-γ, and the chemokine MCP-1, whereas the Br. abortus-infected MG-treated mice revealed enhanced induction of IL-12p70, TNF and IL-10 compared to the MG-untreated control. CONCLUSIONS: MG induced F-actin polymerization and modest upregulation of MAPKs. Furthermore, oral treatment with MG induced an immune response and decreased bacterial proliferation in Br. abortus-infected mice, suggesting that MG may be an alternative treatment for brucellosis. SIGNIFICANCE AND IMPACT OF THE STUDY: The present study demonstrates the therapeutic effects of MG against Brucella infection through induction of cytokine production and protection from bacterial proliferation in the spleens of mice.
AIMS: To investigate the effects of methyl gallate (MG) on murine macrophages, cytokine production and treatment of Brucella abortus infection using a mouse model. METHODS AND RESULTS:MG-treated cells displayed increased F-actin polymerization and modest increase in ERK, JNK and p38α phosphorylation levels. The mice were intraperitoneally infected with Br. abortus and were orally treated with PBS or MG for 14 days. The weight and bacterial number from each spleen were monitored, and the serum was evaluated for cytokine production. The spleen proliferation and bacterial burden were lower in the MG-treated group than in the MG-untreated control. The noninfected MG-treated mice displayed increased production of TNF, IFN-γ, and the chemokine MCP-1, whereas the Br. abortus-infected MG-treated mice revealed enhanced induction of IL-12p70, TNF and IL-10 compared to the MG-untreated control. CONCLUSIONS:MG induced F-actin polymerization and modest upregulation of MAPKs. Furthermore, oral treatment with MG induced an immune response and decreased bacterial proliferation in Br. abortus-infectedmice, suggesting that MG may be an alternative treatment for brucellosis. SIGNIFICANCE AND IMPACT OF THE STUDY: The present study demonstrates the therapeutic effects of MG against Brucella infection through induction of cytokine production and protection from bacterial proliferation in the spleens of mice.
Authors: Gina Porras; François Chassagne; James T Lyles; Lewis Marquez; Micah Dettweiler; Akram M Salam; Tharanga Samarakoon; Sarah Shabih; Darya Raschid Farrokhi; Cassandra L Quave Journal: Chem Rev Date: 2020-11-09 Impact factor: 60.622
Authors: Alisha W B Reyes; Huynh T Hop; Lauren T Arayan; Tran X N Huy; Wongi Min; Hu Jang Lee; Hong Hee Chang; Suk Kim Journal: J Vet Sci Date: 2018-01-31 Impact factor: 1.672
Authors: Dorcas A Gado; Muna Ali Abdalla; Aroke S Ahmed; Balungile Madikizela; Sanah M Nkadimeng; Marthie M Ehlers; Lyndy J McGaw Journal: BMC Complement Med Ther Date: 2021-04-13