M Ruiz-Linares1, B Aguado-Pérez2, P Baca3, M T Arias-Moliz4, C M Ferrer-Luque5. 1. Department of Paediatric Dentistry, University of Granada, Granada, Spain. 2. University of Granada, Granada, Spain. 3. Department of Preventive Dentistry, University of Granada, Granada, Spain. 4. Department of Microbiology, University of Granada, Granada, Spain. 5. Department of Dental Pathology and Therapeutics, School of Dentistry, University of Granada, Granada, Spain.
Abstract
AIM: To evaluate the antibiofilm activity of 2.5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), 2% alexidine (ALX) and 0.2% cetrimide (CTR) alone and in combination on mature polymicrobial root canal biofilms on human dentine using confocal laser scanning microscopy (CLSM). METHODOLOGY: Twenty-eight human dentine specimens were infected for 21 days with microbial samples collected from infected root canals of three volunteers. Antibiofilm activity of the irrigating solutions was evaluated after 3 min of contact time under CLSM. For quantification purposes, bioimage_L software was used. The variables evaluated were the log10 of total biovolume (μm3 ) and percentage of live cells (green) population. Statistical analysis of both variables was performed using an anova test and a post hoc Duncan test to determine significant clusters amongst groups. The variable green population percentage was previously subjected to the normalized Anscombe transformation. RESULTS: The NaOCl group had a total biovolume and percentage of live cells significantly lower than the other groups (P ˂ 0.001). The addition of 0.2% CTR significantly increased the antimicrobial effect of 2% CHX (P ˂ 0.001). There were no significant differences between 0.2% CTR, 2% ALX and the combination of both (P ˂ 0.05). CONCLUSIONS: Overall, 2.5% NaOCl dissolved and killed bacteria significantly more efficiently when used against polymicrobial mature biofilm on human dentine. Cetrimide improved the antimicrobial activity of chlorhexidine and alexidine.
AIM: To evaluate the antibiofilm activity of 2.5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), 2% alexidine (ALX) and 0.2% cetrimide (CTR) alone and in combination on mature polymicrobial root canal biofilms on human dentine using confocal laser scanning microscopy (CLSM). METHODOLOGY: Twenty-eight human dentine specimens were infected for 21 days with microbial samples collected from infected root canals of three volunteers. Antibiofilm activity of the irrigating solutions was evaluated after 3 min of contact time under CLSM. For quantification purposes, bioimage_L software was used. The variables evaluated were the log10 of total biovolume (μm3 ) and percentage of live cells (green) population. Statistical analysis of both variables was performed using an anova test and a post hoc Duncan test to determine significant clusters amongst groups. The variable green population percentage was previously subjected to the normalized Anscombe transformation. RESULTS: The NaOCl group had a total biovolume and percentage of live cells significantly lower than the other groups (P ˂ 0.001). The addition of 0.2% CTR significantly increased the antimicrobial effect of 2% CHX (P ˂ 0.001). There were no significant differences between 0.2% CTR, 2% ALX and the combination of both (P ˂ 0.05). CONCLUSIONS: Overall, 2.5% NaOCl dissolved and killed bacteria significantly more efficiently when used against polymicrobial mature biofilm on human dentine. Cetrimide improved the antimicrobial activity of chlorhexidine and alexidine.
Authors: Adriana Oliveira; Daniel Rocha; Alexandre De Martin; Ana-Grasiela Limoeiro; Wayne Nascimento; Carlos Fontana; Rina Pelegrine; Elizabeth Martinez; Carlos Bueno Journal: J Clin Exp Dent Date: 2022-08-01